Saed Ghassan M, Diamond Michael P
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, and the C. S. Mott Center for Human Growth and Development, Wayne State University, Detroit, Michigan 48201, USA.
Fertil Steril. 2003 Jan;79(1):164-8. doi: 10.1016/s0015-0282(02)04557-0.
To determine whether normal peritoneal and adhesion fibroblasts express tissue plasminogen activator (tPA) and plasminogen activator inhibitor (PAI-I) and whether their expression is regulated by oxygen.
Prospective experimental study.
University medical center.
PATIENT(S): Cultures of human fibroblasts established from peritoneal and adhesion tissues. Hypoxia treatment of the primary cultured fibroblasts.
MAIN OUTCOME MEASURE(S): We have used the multiplex reverse transcription polymerase chain reaction (RT-PCR) technique to determine the effect of hypoxia on the expression of tPA and PAI-I in normal peritoneal (NPF) and adhesion (ADF) fibroblasts. Cultures of NPF and ADF were exposed to hypoxia (2% O(2)) for 24 hours. RNA was extracted from cells and subjected to multiplex RT-PCR to quantitate relative changes in mRNA levels of tPA and PAI-I in response to hypoxia treatment.
RESULT(S): Basal tPA mRNA levels are present in both NPF and ADF and were 45% higher in NPF than ADF. Hypoxia decreased tPA in both NPF and ADF by 74% and 95%, respectively. Basal PAI-I mRNA levels were 64% higher in ADF than in NPF. Hypoxia increased PAI-I mRNA levels by 67% and 53% in NPF and ADF, respectively.
CONCLUSION(S): Plasminogen activator activity (PAA) of the peritoneum does not solely reside in the mesothelial cells, as previously identified, but also exists within fibroblasts, thus providing the potential to resolve postoperative fibrinous collections even at sites at which the mesothelial cells have been injured, removed, or destroyed. Furthermore, PAA in fibroblasts is regulated by oxygen; creation of a hypoxic state markedly attenuates PAA, thereby leading to adhesion development.
确定正常腹膜和粘连成纤维细胞是否表达组织型纤溶酶原激活物（tPA）和纤溶酶原激活物抑制剂（PAI-I），以及它们的表达是否受氧调节。
前瞻性实验研究。
大学医学中心。
从腹膜和粘连组织建立的人成纤维细胞培养物。对原代培养的成纤维细胞进行缺氧处理。
我们使用多重逆转录聚合酶链反应（RT-PCR）技术来确定缺氧对正常腹膜（NPF）和粘连（ADF）成纤维细胞中tPA和PAI-I表达的影响。将NPF和ADF培养物暴露于缺氧（2% O₂）环境24小时。从细胞中提取RNA，并进行多重RT-PCR以定量缺氧处理后tPA和PAI-I mRNA水平的相对变化。
NPF和ADF中均存在基础tPA mRNA水平，且NPF中的基础tPA mRNA水平比ADF高45%。缺氧分别使NPF和ADF中的tPA降低了74%和95%。ADF中的基础PAI-I mRNA水平比NPF高64%。缺氧使NPF和ADF中的PAI-I mRNA水平分别增加了67%和53%。
腹膜的纤溶酶原激活物活性（PAA）并不像之前所认为的那样仅存在于间皮细胞中，还存在于成纤维细胞内，因此即使在间皮细胞已受损、被移除或被破坏的部位，也有解决术后纤维蛋白性渗出物的潜力。此外，成纤维细胞中的PAA受氧调节；低氧状态的形成会显著减弱PAA，从而导致粘连形成。
