[人参农家品种的扩增片段长度多态性指纹图谱研究]
[A study on AFLP fingerprinting of land races of Panax ginseng L].
作者信息
Ma X J, Wang X Q, Xiao P G, Hong D Y
机构信息
Institute of Medicinal Plant, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100094, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2000 Dec;25(12):707-10.
OBJECTIVE
To obtain more information on DNA fingerprintings of five land races of Chinese ginseng, namely, Damaya (DMY), Changbo (CB), Yuanbangyuanlu (YBYL) and Huangguo (HG).
METHODS
The five land races were detected by amplified restriction fragment polymorphism (AFLP) markers with 11 combined primers (M2, M3, M16, M20, M53, M56, M57, M68, M69, M72, M84 in Mse I).
RESULT AND CONCLUSION
Only 4.6% polymorphic sites was found. It was further verified that only a little diversity existed among the land races. The polymorphic sites of CB were much more than those of the others, which suggests that there are more heterozygotes in CB populations, and it is closer to wild ginseng than the others.
目的
获取更多关于中国人参五个农家品种大马牙（DMY）、长脖（CB）、圆膀圆芦（YBYL）和黄果（HG）的DNA指纹图谱信息。
方法
使用11对组合引物（Mse I中的M2、M3、M16、M20、M53、M56、M57、M68、M69、M72、M84）通过扩增限制性片段多态性（AFLP）标记对这五个农家品种进行检测。
结果与结论
仅发现4.6%的多态性位点。进一步证实这些农家品种之间仅存在少量差异。CB的多态性位点比其他品种多得多，这表明CB群体中有更多杂合子，且它比其他品种更接近野生人参。
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