Cui Guang-hong, Huang Lu-qi, Tang Xiao-jing, He Xi-rong, Li Xin
Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2007 Jun;32(11):1012-5.
Searching a new molecular method to authenticate Panax ginseng and P. quenquefolium.
Single primers based on rDNA sequences of Panax species were designed to obtain polymorphic bands of P. ginseng and P. quinquefolius and then sequenced. Four PCR primers (two forword and two reverse primers) specific to P. ginseng and P. quinquefolius were designed.
Primer Pg-6F, Pg-479R only amplified 474 bp band for P. ginseng and primer Pq-442F, Pq-658R only amplified 217 bp band for P. quinquefolius. It is indicated that the four primers could serve as specific STS primers for Panax species.
A new way to obtain STS primers of Panax species was established. This method is more quick and efficient than SCAR-PCR method and can serve as a model to obtain molecular markers for other Chinese material medica.
寻找一种鉴定人参和西洋参的新分子方法。
基于人参属物种的rDNA序列设计单引物，以获得人参和西洋参的多态性条带，然后进行测序。设计了4条针对人参和西洋参的PCR引物（2条正向引物和2条反向引物）。
引物Pg-6F、Pg-479R仅为人参扩增出474 bp的条带，引物Pq-442F、Pq-658R仅为西洋参扩增出217 bp的条带。表明这4条引物可作为人参属物种的特异性STS引物。
建立了一种获得人参属物种STS引物的新方法。该方法比SCAR-PCR方法更快速、高效，可作为获取其他中药材分子标记的模型。
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