[Establishment and application of AFLP fingerprinting system in Atractylodes macrocephala germplasm].

OBJECTIVE

To establish the AFLP Fingerprinting system in the germplasm of Atractylodes macrocephala Koidz.

METHODS

10 wild or cultivated Atractylodes macrocephala were used for AFLP fingerprinting analysis by EcoRI and MseI restriction enzymes with silver staining.

RESULTS

Using 2X CTAB buffer extraction method can obtain the best genomic DNA samples. According to the AFLP polymorphism, sixteen out of forty primer pairs were selected to be suitable for AFLP analysis. Total 3003 polymorphic bands were obtained from the 16 sets of primer. Based on the AFLP results, the 10 samples of Atractylodes macrocephala germplasm were classified into four types.

CONCLUSION

The establishment of the AFLP fingerprinting system in Atractylodes macrocephala will be used in the identification of germplasms and breeding of the species.