Wang Zhi-An, Xu Xing, Shen Xiao-Xia, Chen Bin-Long, Shou Hui-Xia
Zhejiang Institute of Chinese Meteria Medica, Hangzhou 310023, China.
Zhong Yao Cai. 2008 Apr;31(4):483-7.
To establish the AFLP Fingerprinting system in the germplasm of Atractylodes macrocephala Koidz.
10 wild or cultivated Atractylodes macrocephala were used for AFLP fingerprinting analysis by EcoRI and MseI restriction enzymes with silver staining.
Using 2X CTAB buffer extraction method can obtain the best genomic DNA samples. According to the AFLP polymorphism, sixteen out of forty primer pairs were selected to be suitable for AFLP analysis. Total 3003 polymorphic bands were obtained from the 16 sets of primer. Based on the AFLP results, the 10 samples of Atractylodes macrocephala germplasm were classified into four types.
The establishment of the AFLP fingerprinting system in Atractylodes macrocephala will be used in the identification of germplasms and breeding of the species.
建立白术种质的AFLP指纹图谱体系。
选用10份野生或栽培白术，采用EcoRI和MseI限制性内切酶进行AFLP指纹图谱分析，银染法显色。
采用2X CTAB缓冲液提取法可获得最佳基因组DNA样本。根据AFLP多态性，从40对引物中筛选出16对适合AFLP分析的引物。16组引物共获得3003条多态性条带。基于AFLP分析结果，将10份白术种质样本分为4类。
白术AFLP指纹图谱体系的建立将用于该物种的种质鉴定和育种。
