Li Z X, Wang X H, Zhao J H, Yang J F, Wang X
Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei, China.
Zhongguo Zhong Yao Za Zhi. 2000 Dec;25(12):742-5.
To evaluate the antibacterial activity of Forsythia suspensa in vitro with different media.
MIC determination of Forsythia suspensa against Staphylococci was performed by the agar dilution method.
MIC90 of decoction of Forsythia suspensa against Staphylococcus epidermidis in M-H agar was 1:640, but in nutrient agar 1:40, the antibacterial activity with M-H agar being 16 fold higher than nutrient agar.
The M-H agar should be recommended to replace nutrient agar as medium in the antibacterial experiment of Traditional Chinese medicine, and it is better to use multipoint inoculating device in the sensitivity test.
评价不同培养基条件下连翘的体外抗菌活性。
采用琼脂稀释法测定连翘对葡萄球菌的最低抑菌浓度（MIC）。
连翘水煎液对表皮葡萄球菌在M-H琼脂中的MIC90为1:640，在营养琼脂中为1:40，在M-H琼脂中的抗菌活性比营养琼脂高16倍。
在中药抗菌实验中，推荐使用M-H琼脂替代营养琼脂作为培养基，且在药敏试验中最好使用多点接种装置。
