Qin Qun, Xie Zhao-xia, Pi Hai-zhen
Department of Pharmacy, Xiangya Hospital, Central South University, Changsha 410008, China.
Hunan Yi Ke Da Xue Xue Bao. 2002 Feb 28;27(1):69-70.
To study the antibacterial activity of Chinese medicine kou-Kang mouthwash and its acute toxicity, and provide the basis of experiment and therapy for infectious diseases of the oral cavity.
Minimal inhibitory concentrations (MIC) of kou-Kang mouthwash were determined by the agar dilution method, and the acute toxicity was done.
The range of MIC kou-Kang mouthwash against 27 Gram negative bacterial were 0.03-1.0 g.ml-1. MIC50 was 0.125 g.ml-1 and MIC90 was 0.125-0.5 g.ml-1. The range of MIC kou-Kang mouthwash against 21 Gram positive bacterial was 0.008-0.5 g.ml-1. The range of MIC50 and MIC90 were 0.015-0.125 g.ml-1 and 0.015-0.5 g.ml-1, respectively. The value of MIC was 0.015 g.ml-1 against candidia albicans. The acute toxic test indicated that the maximum tolerance dose was 417.6 g.kg-1 and 210 times as much as the clinical dose a day. No death of animals or toxicity and side-effect occurred in the test.
Kou-Kang mouthwash shows a potent antibacterial activity against various pathogens of the oral cavity. Its antibiotic activity against Gram positive bacteria was 2-8 times stronger than that against Gram negative bacteria. It has low toxicity and can be used in clinical application.
研究中药口康漱口液的抗菌活性及其急性毒性，为口腔感染性疾病提供实验及治疗依据。
采用琼脂稀释法测定口康漱口液的最低抑菌浓度（MIC），并进行急性毒性试验。
口康漱口液对27种革兰阴性菌的MIC范围为0.03～1.0g·ml-1，MIC50为0.125g·ml-1，MIC90为0.125～0.5g·ml-1。对21种革兰阳性菌的MIC范围为0.008～0.5g·ml-1，MIC50和MIC90范围分别为0.015～0.125g·ml-1和0.015～0.5g·ml-1。对白假丝酵母菌的MIC值为0.015g·ml-1。急性毒性试验表明，最大耐受剂量为417.6g·kg-1，是临床一日剂量的210倍。试验中动物无死亡，未出现毒性及不良反应。
口康漱口液对口腔多种病原菌具有较强的抗菌活性。其对革兰阳性菌的抗菌活性比对革兰阴性菌强2～8倍。毒性低，可用于临床。
