Pasquier Christophe, Bujan Louis, Daudin Myriam, Righi Laurence, Berges Laetitia, Thauvin Laurence, Berrebi Alain, Massip Patrice, Puel Jacqueline, Izopet Jacques
Laboratoire de Virologie, UFR des Sciences Pharmaceutiques et Faculté de Médecine de Toulouse-Purpan, Hôpital Purpan, Toulouse, France.
J Med Virol. 2003 Mar;69(3):344-9. doi: 10.1002/jmv.10295.
HCV is usually transmitted via the blood, but HCV RNA has been detected recently in seminal fluid. This study was done to study HCV seminal shedding and factors that could influence the presence of HCV in the seminal fluid of men coinfected with HCV and HIV-1. HCV and HIV-1 genomes were assayed in multiple paired blood and semen samples obtained from 35 men enrolled in an assisted medical procreation protocol. HCV RNA was found intermittently in semen samples from 9 patients (25.7%). Samples from 9 men with HCV RNA in their semen and 26 men without were compared to further analyze these parameters. No correlation was found between HCV RNA in the seminal fluid and age, HCV virus load, the duration of HIV-1 infection, HIV treatment, the CD4(+) cell count, HIV-1 virus load or HIV-1 detection in the semen. The intermittent detection of HCV RNA in semen samples support the systematic search for HCV RNA in semen and the use of processed spermatozoa in assisted medical procreation of infertile HCV serodiscordant couples.
丙型肝炎病毒（HCV）通常通过血液传播，但最近在精液中检测到了HCV RNA。本研究旨在研究HCV精液排出情况以及可能影响合并感染HCV和HIV-1的男性精液中HCV存在的因素。对35名参与辅助医疗生殖方案的男性采集的多对配对血液和精液样本进行了HCV和HIV-1基因组检测。在9名患者（25.7%）的精液样本中间歇性地检测到HCV RNA。对9名精液中存在HCV RNA的男性和26名精液中不存在HCV RNA的男性的样本进行比较，以进一步分析这些参数。精液中的HCV RNA与年龄、HCV病毒载量、HIV-1感染持续时间、HIV治疗、CD4(+)细胞计数、HIV-1病毒载量或精液中HIV-1检测之间均未发现相关性。精液样本中HCV RNA的间歇性检测支持在精液中系统检测HCV RNA，并在不育的HCV血清学不一致夫妇的辅助医疗生殖中使用处理过的精子。
