Forman J, Klein J
J Immunol. 1975 Sep;115(3):711-5.
The H-2da haplotype was derived from the H-2d haplotype by a mutation localized to the D end of the H-2 complex. Coculture of H-2d and H-2da spleen cells gives rise to bidirectional MLR. However, the H-2d anti-H-2da response is much stronger than that of H-2da anti-H-2d. Both haplotypes give rise to reciprocal CML. B10.D2(R103) strain spleen cells, which differ only at the D end of the H-2 complex from the H-2d haplotype, kill H-2da target cells in CML when sensitized to H-2d stimulators and vice versa. Therefore, both the mutant and strain of origin share a D end CML specificity. H-2d and H-2da reject skin grafts in both directions, although some H-2d grafts show prolonged acceptance on H-2da recipients. These data are consistent with a mutation in the D end of the H-2d haplotype resulting in gain-loss of an antigen(s) that gives rise to reciprocal MLR, CML, and skin graft rejection. Further, the mutant can be distinguished from the strain of origin on the basis of the strength of immune response in MLR.
H-2da单倍型由H-2d单倍型通过定位在H-2复合体D端的一个突变产生。H-2d和H-2da脾细胞共培养会引发双向混合淋巴细胞反应（MLR）。然而，H-2d抗H-2da反应比H-2da抗H-2d反应强得多。两种单倍型都会引发相互的细胞介导的淋巴细胞毒反应（CML）。B10.D2(R103)品系的脾细胞，其在H-2复合体的D端与H-2d单倍型仅有差异，当被H-2d刺激细胞致敏时，在CML中能杀伤H-2da靶细胞，反之亦然。因此，突变体及其起源品系共享D端CML特异性。H-2d和H-2da在两个方向上都能排斥皮肤移植，尽管一些H-2d移植在H-2da受体上显示出较长的存活期。这些数据与H-2d单倍型D端的一个突变导致一种抗原的得失一致，该抗原引发相互的MLR、CML和皮肤移植排斥。此外，基于MLR中免疫反应的强度，可以将突变体与起源品系区分开来。
