Nowell P C, Finan J B, Wilson D B
J Exp Med. 1975 Jul 1;142(1):230-5. doi: 10.1084/jem.142.1.230.
Rat lymphocytes in mixed cultures can reutilize tritiated thymidine from labeled granulocytes. Shortly after thymidine injections in vivo, major effects on the frequency of labeled lymphocyte mitoses in peripheral blood cultures are introduced by 10-20% polymorph contamination, even though transfer of label via supernates is not demonstrable. Cold thymidine in the cultures prevents reutilization, and has permitted reevaluation of several previous conclusions concerning the life history of lymphocytes reactive to major histocompatibility alloantigens (HARC). Rather than being predominantly recently divided cells, HARC do not appear to have an age distribution, in blood or lymph, significantly different from the general recirculating lymphocyte population. However, the ability of immunization across strong allogeneic differences to increase markedly the proportion of young HARC among the specifically responsive population has been confirmed.
混合培养中的大鼠淋巴细胞可以重新利用来自标记粒细胞的氚化胸腺嘧啶核苷。在体内注射胸腺嘧啶核苷后不久，即使通过上清液的标记转移无法证实，10 - 20%的多形核细胞污染也会对外周血培养中标记淋巴细胞有丝分裂的频率产生主要影响。培养物中的冷胸腺嘧啶核苷可阻止重新利用，并使得对先前有关对主要组织相容性同种异体抗原（HARC）有反应的淋巴细胞的生命史的几个结论进行重新评估。HARC并非主要是最近分裂的细胞，在血液或淋巴中，其年龄分布与一般再循环淋巴细胞群体相比似乎没有显著差异。然而，通过强烈同种异体差异进行免疫以显著增加特异性反应群体中年轻HARC比例的能力已得到证实。
