灵长类外侧膝状体核大细胞层中神经丝蛋白M'和αβ-晶体蛋白的免疫组织化学研究。

Immunohistochemical investigations of neurofilament M' and alphabeta-crystallin in the magnocellular layers of the primate lateral geniculate nucleus.

作者信息

Prasad S S, Schnerch A, Lam D Y, To E, Jim J, Kaufman P L, Matsubara J A

机构信息

Department of Ophthalmology and Visual Sciences, University of British Columbia, 2550 Willow Street, Vancouver, British Columbia, Canada V5Z 3N9.

出版信息

Brain Res Mol Brain Res. 2002 Dec 30;109(1-2):216-20. doi: 10.1016/s0169-328x(02)00564-8.

DOI:10.1016/s0169-328x(02)00564-8

PMID:12531531

Abstract

The magnocellular and parvocellular pathways are two major processing streams in the primate visual system. Using high-density grid arrayed cDNA clones to hybridize to cDNA probes from cortical regions of each pathway, a list of candidate differentially expressed genes was produced [Mol. Brain Res. 82 (2000) 11-24]. Magnocellular pathway candidates include neurofilament M' and alphabeta-crystallin. Using antibodies generated against these proteins, immunohistochemical analysis revealed preferential staining of the magnocellular layers in the primate lateral geniculate nucleus, providing verification of two candidate magnocellular-enriched genes.

摘要

大细胞通路和小细胞通路是灵长类视觉系统中的两条主要处理流。利用高密度网格排列的cDNA克隆与来自每条通路皮质区域的cDNA探针杂交，生成了一份候选差异表达基因列表[《分子脑研究》82(2000)11 - 24]。大细胞通路候选基因包括神经丝蛋白M'和αβ-晶状体蛋白。使用针对这些蛋白质产生的抗体，免疫组织化学分析显示在灵长类外侧膝状核的大细胞层有优先染色，证实了两个候选的富含大细胞的基因。