Chaudhuri A, Zangenehpour S, Matsubara J A, Cynader M S
Department of Ophthalmology, University of British Columbia, Vancouver, Canad
Brain Res. 1996 Feb 12;709(1):17-26. doi: 10.1016/0006-8993(95)01217-6.
It has been previously reported that the monoclonal antibody SMI-32 reveals a characteristic pattern of immunostaining which may be used to delineate various cortical modules in the monkey visual system. We wished to examine staining patterns with this antibody at both the lateral geniculate nucleus (LGN) and cortical levels with regard to magno- and parvocellular processing schemes in the vervet monkey. Using standard immunohistochemical procedures, we have found that the M-layers of the LGN were intensely stained in comparison to P-layers and that there were regional variations in staining within the visual cortex that reflected this input. The transition between areas V1 and V2 was especially prominent due to differences in the laminar staining profiles. Another striking result was found within the superior temporal sulcus where heavy SMI-32 immunostaining confined to the floor of the sulcus coincided with a similar zone of intense myelin staining. We have also found a number of other areas within the intraparietal and lateral sulci that show foci of heavy SMI-32 staining. As with Cat-301 immunostaining, the regional variabilities that are observed with SMI-32 in the visual cortex reflect molecular distinctions that may provide further criteria for functional segmentation.
此前有报道称，单克隆抗体SMI-32显示出一种特征性的免疫染色模式，可用于描绘猕猴视觉系统中的各种皮质模块。我们希望研究该抗体在外侧膝状体核（LGN）和皮质水平上的染色模式，以了解绿猴的大细胞和小细胞处理方案。使用标准免疫组织化学程序，我们发现LGN的M层与P层相比染色强烈，并且视觉皮质内的染色存在区域差异，反映了这种输入。由于层状染色轮廓的差异，V1和V2区域之间的过渡尤为突出。在颞上沟内还发现了另一个惊人的结果，即局限于沟底的强烈SMI-32免疫染色与类似的强烈髓鞘染色区域相吻合。我们还在内顶叶沟和外侧沟内发现了许多其他区域，这些区域显示出强烈的SMI-32染色焦点。与Cat-301免疫染色一样，在视觉皮质中观察到的SMI-32区域变异性反映了分子差异，这可能为功能分割提供进一步的标准。
