Vickers J C, Hof P R, Schumer R A, Wang R F, Podos S M, Morrison J H
Division of Pathology, Clinical School, University of Tasmania, Hobart, Australia.
Aust N Z J Ophthalmol. 1997 Aug;25(3):239-43. doi: 10.1111/j.1442-9071.1997.tb01400.x.
Neurochemical changes in nerve cells were investigated in the lateral geniculate nucleus (LGN) and primary visual cortex of macaque monkeys with experimentally induced glaucoma.
Glaucomatous damage was induced in one eye of experimental animals by elevation of intraocular pressure following laser burns to the trabecular meshwork. Staining for the metabolic marker cytochrome oxidase, as well as immunolabelling for the neuronal markers synaptophysin and neurofilament proteins, was conducted on sections of the LGN and primary visual cortex.
In the LGN, staining for cytochrome oxidase and immunolabelling for synaptophysin were reduced in the parvocellular and magnocellular layers that received input from the glaucomatous eye and neurofilament protein labelling was reduced in the parvocellular layers. Cytochrome oxidase staining demonstrated the presence of denervated ocular dominance columns in layer IVC of the primary visual cortex of experimental animals.
Pre- and post-synaptic neurochemical alterations in the magnocellular and parvocellular visual pathways of the brain are associated with experimentally induced glaucoma in macaque monkeys.
研究实验性诱导青光眼的猕猴外侧膝状体（LGN）和初级视皮层神经细胞中的神经化学变化。
通过对小梁网进行激光烧灼后升高眼压，在实验动物的一只眼睛中诱导青光眼性损伤。对LGN和初级视皮层切片进行代谢标记细胞色素氧化酶染色以及神经元标记突触素和神经丝蛋白的免疫标记。
在LGN中，接受来自青光眼眼输入的小细胞层和大细胞层中细胞色素氧化酶染色和突触素免疫标记减少，小细胞层中神经丝蛋白标记减少。细胞色素氧化酶染色显示实验动物初级视皮层IVC层中存在去神经支配的眼优势柱。
大脑大细胞和小细胞视觉通路中突触前和突触后的神经化学改变与猕猴实验性诱导的青光眼有关。
