一种感染桃蚜的新型病毒，其基因组结构与浓核病毒属的物种相似。

A new virus infecting Myzus persicae has a genome organization similar to the species of the genus Densovirus.

作者信息

van Munster M, Dullemans A M, Verbeek M, van den Heuvel J F J M, Reinbold C, Brault V, Clérivet A, van der Wilk F

机构信息

Plant Research International, PO Box 16, 6700 AA Wageningen, The Netherlands.

De Ruiter Seeds, PO Box 1050, 2660 BB Bergschenhoek, The Netherlands.

出版信息

J Gen Virol. 2003 Jan;84(Pt 1):165-172. doi: 10.1099/vir.0.18650-0.

DOI:10.1099/vir.0.18650-0

PMID:12533713

Abstract

The genomic sequence of a new icosahedral DNA virus infecting Myzus persicae has been determined. Analysis of 5499 nt of the viral genome revealed five open reading frames (ORFs) evenly distributed in the 5' half of both DNA strands. Three ORFs (ORF1-3) share the same strand, while two other ORFs (ORF4 and ORF5) are detected in the complementary sequence. The overall genomic organization is similar to that of species from the genus DENSOVIRUS: ORFs 1-3 most likely encode the non-structural proteins, since their putative products contain conserved replication motifs, NTP-binding domains and helicase domains similar to those found in the NS-1 protein of parvoviruses. The deduced amino acid sequences from ORFs 4 and 5 show sequence similarities with the structural proteins of the members of the genus DENSOVIRUS: These data indicate that this virus is a new species of the genus Densovirus in the family PARVOVIRIDAE: The virus was tentatively named Myzus persicae densovirus.

摘要

一种感染桃蚜的新型二十面体DNA病毒的基因组序列已被测定。对病毒基因组5499个核苷酸的分析揭示了五个开放阅读框（ORF），它们均匀分布在两条DNA链的5'端。三个ORF（ORF1 - 3）位于同一条链上，而另外两个ORF（ORF4和ORF5）在互补序列中被检测到。整体基因组结构与浓核病毒属的物种相似：ORF1 - 3很可能编码非结构蛋白，因为它们的推定产物包含保守的复制基序、NTP结合结构域和解旋酶结构域，类似于细小病毒NS - 1蛋白中的结构域。从ORF4和ORF5推导的氨基酸序列与浓核病毒属成员的结构蛋白显示出序列相似性：这些数据表明该病毒是细小病毒科浓核病毒属的一个新物种：该病毒被暂时命名为桃蚜浓核病毒。