Al-Adham I S I, Al-Hmoud N D, Khalil E, Kierans M, Collier P J
School of Pharmacy & Medical Technology, University of Petra, Amman, Jordan, School of Contemporary Sciences, University of Abertay Dundee, Dundee, Scotland, UK.
Lett Appl Microbiol. 2003;36(2):97-100. doi: 10.1046/j.1472-765x.2003.01266.x.
The demonstration of the antibiofilm effects of pharmaceutical microemulsions.
Microemulsions were prepared as physically stable oil/water systems. Previous work by this group has shown that microemulsions are highly effective antimembrane agents that result in rapid losses of viability in planktonic populations of Pseudomonas aeruginosa and Staphylococcus aureus. In this experiment a microemulsion preparation was used upon established biofilm cultures of Ps. aeruginosa PA01 for a period of 4 h. The planktonic MIC of sodium pyrithione and the planktonic and biofilm MICs of cetrimide were used as positive controls and a biofilm was exposed to a volume of normal sterile saline as a treatment (negative) control. Results indicate three log-cycle reductions in viability within the microemulsion treated biofilm, as compared to those observed in control treatments of similar biofilms (one log-cycle reduction in viabilities).
The results indicate that the microemulsions are highly effective antibiofilm agents.
This study suggests that microemulsions may have a role in the treatment of industrial and environmental biofilms.
证明药用微乳剂的抗生物膜作用。
将微乳体制备成物理稳定的油/水体系。该研究小组之前的工作表明，微乳剂是高效的抗膜剂，能使铜绿假单胞菌和金黄色葡萄球菌浮游菌群体的活力迅速丧失。在本实验中，将一种微乳剂制剂作用于已形成的铜绿假单胞菌PA01生物膜培养物4小时。用吡硫翁钠的浮游菌最低抑菌浓度（MIC）以及西曲溴铵的浮游菌和生物膜MIC作为阳性对照，将生物膜暴露于一定体积的普通无菌盐水中作为处理（阴性）对照。结果表明，与类似生物膜的对照处理相比（活力降低一个对数周期），微乳剂处理的生物膜活力降低了三个对数周期。
结果表明微乳剂是高效的抗生物膜剂。
本研究表明微乳剂可能在工业和环境生物膜的治疗中发挥作用。
