Whitham Steven A, Quan Sheng, Chang Hur-Song, Cooper Bret, Estes Bram, Zhu Tong, Wang Xun, Hou Yu-Ming
Department of Plant Pathology, Iowa State University, Ames, IA 50011-1020, USA.
Plant J. 2003 Jan;33(2):271-83. doi: 10.1046/j.1365-313x.2003.01625.x.
Systemic infections of plants by viruses require that viruses modify host cells in order to facilitate infections. These modifications include induction of host factors required for replication, propagation and movement, and suppression of host defense responses, which are likely to be associated with changes in host gene expression. Past studies of the effects of viral infection on gene expression in susceptible hosts have been limited to only a handful of genes. To gain broader insight into the responses elicited by viruses in susceptible hosts, high-density oligonucleotide probe microarray technology was used. Arabidopsis leaves were either mock inoculated or inoculated with cucumber mosaic cucumovirus, oil seed rape tobamovirus, turnip vein clearing tobamovirus, potato virus X potexvirus, or turnip mosaic potyvirus. Inoculated leaves were collected at 1, 2, 4, and 5 days after inoculation, total RNA was isolated, and samples were hybridized to Arabidopsis GeneChip microarrays (Affymetrix). Microarray hybridization revealed co-ordinated changes in gene expression in response to infection by diverse viruses. These changes include virus-general and virus-specific alterations in the expression of genes associated with distinct defense or stress responses. Analyses of the promoters of these genes further suggest that diverse RNA viruses elicit common responses in susceptible plant hosts through signaling pathways that have not been previously characterized.
植物受到病毒的系统性感染需要病毒对宿主细胞进行修饰，以促进感染。这些修饰包括诱导复制、传播和移动所需的宿主因子，以及抑制宿主防御反应，而这可能与宿主基因表达的变化有关。过去关于病毒感染对易感宿主基因表达影响的研究仅限于少数几个基因。为了更全面地了解病毒在易感宿主中引发的反应，我们使用了高密度寡核苷酸探针微阵列技术。拟南芥叶片要么进行模拟接种要么接种黄瓜花叶黄瓜病毒、油菜烟草花叶病毒、芜菁脉明烟草花叶病毒、马铃薯X病毒或芜菁花叶马铃薯Y病毒。在接种后的第1、2、4和5天收集接种的叶片，分离总RNA，并将样品与拟南芥基因芯片微阵列（Affymetrix）杂交。微阵列杂交揭示了基因表达的协同变化以响应不同病毒的感染。这些变化包括与不同防御或应激反应相关基因表达中的病毒共性和病毒特异性改变。对这些基因启动子的分析进一步表明，多种RNA病毒通过以前未被表征的信号通路在易感植物宿主中引发共同反应。
