Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK.
Alliance of Bioversity International and International Center for Tropical Agriculture (CIAT), Dong Dok, Ban Nongviengkham, Vientiane CB10 1RQ, Laos.
Viruses. 2022 Aug 1;14(8):1703. doi: 10.3390/v14081703.
Cucumber mosaic virus (CMV), a major tomato pathogen, is aphid-vectored in the non-persistent manner. We investigated if CMV-induced volatile organic compounds (VOCs) or other virus-induced cues alter aphid-tomato interactions. Y-tube olfactometry showed that VOCs emitted by plants infected with CMV (strain Fny) attracted generalist () and Solanaceae specialist () aphids. preferred settling on infected plants (3 days post-inoculation: dpi) at 1h post-release, but at 9 and 21 dpi, aphids preferentially settled on mock-inoculated plants. showed no strong preference for mock-inoculated versus infected plants at 3 dpi but settled preferentially on mock-inoculated plants at 9 and 21 dpi. In darkness aphids showed no settling or migration bias towards either mock-inoculated or infected plants. However, tomato VOC blends differed in light and darkness, suggesting aphids respond to a complex mix of olfactory, visual, and other cues influenced by infection. The LS-CMV strain induced no changes in aphid-plant interactions. Experiments using inter-strain recombinant and pseudorecombinant viruses showed that the Fny-CMV 2a and 2b proteins modified tomato interactions with and , respectively. The defence signal salicylic acid prevents excessive CMV-induced damage to tomato plants but is not involved in CMV-induced changes in aphid-plant interactions.
黄瓜花叶病毒（CMV）是一种主要的番茄病原体，以非持久方式通过蚜虫传播。我们研究了 CMV 诱导的挥发性有机化合物（VOCs）或其他病毒诱导的线索是否改变了蚜虫-番茄的相互作用。Y 型嗅觉仪检测表明，感染 CMV（Fny 株）的植物释放的 VOCs 吸引了多食性（）和茄科专食性（）蚜虫。在释放后 1 小时，更喜欢定殖在感染植物上（接种后 3 天：dpi），但在 9 和 21 dpi，蚜虫更倾向于定殖在模拟接种的植物上。在 3 dpi 时，对模拟接种的植物与感染植物没有强烈的偏好，但在 9 和 21 dpi 时更倾向于定殖在模拟接种的植物上。在黑暗中，蚜虫对模拟接种的植物和感染植物没有定居或迁移的偏好。然而，番茄 VOC 混合物在光照和黑暗中存在差异，这表明蚜虫对嗅觉、视觉和其他受感染影响的线索的复杂组合做出反应。LS-CMV 株不会改变蚜虫与植物的相互作用。使用株间重组和假重组病毒的实验表明，Fny-CMV 2a 和 2b 蛋白分别修饰了番茄与和的相互作用。防御信号水杨酸可防止 CMV 诱导的番茄过度损伤，但不参与 CMV 诱导的蚜虫-植物相互作用的改变。
