Palacios Gabriel, Muro Asunción, Vela José Miguel, Molina-Holgado Eduardo, Guitart Xavier, Ovalle Sergio, Zamanillo Daniel
Histopathology Unit, Research Center, Laboratorios del Dr. Esteve, S.A., Av. Mare de Déu de Montserrat 221, 08041 Barcelona, Spain.
Brain Res. 2003 Jan 24;961(1):92-9. doi: 10.1016/s0006-8993(02)03892-1.
By using a new polyclonal antibody raised against a 21-amino acid peptide sequence corresponding to the fragment 138-157 of the cloned rat sigma(1)-receptor, we demonstrated by immunoperoxidase and double immunofluorescence techniques, that rat oligodendrocytes express the sigma(1)-receptor. Experiments in vivo and in vitro showed that sigma(1)-receptor colocalized with specific markers of progenitor (A2B5) and mature oligodendrocytes (GalC, RIP). These results suggest that sigma(1)-receptor in oligodendrocytes might be involved in myelination by direct implication in cholesterol biosynthesis or by interaction with endogenous ligands such as neurosteroids.
通过使用针对与克隆的大鼠σ1受体的138 - 157片段相对应的21个氨基酸肽序列产生的新型多克隆抗体，我们通过免疫过氧化物酶和双重免疫荧光技术证明，大鼠少突胶质细胞表达σ1受体。体内和体外实验表明，σ1受体与祖细胞（A2B5）和成熟少突胶质细胞（GalC、RIP）的特异性标志物共定位。这些结果表明，少突胶质细胞中的σ1受体可能通过直接参与胆固醇生物合成或与内源性配体如神经甾体相互作用而参与髓鞘形成。
