Palacios Gabriel, Muro Asunción, Verdú Enric, Pumarola Martí, Vela José Miguel
Histopathology Unit, Research Center, Laboratorios del Dr. Esteve, S.A., Av. Mare de Déu de Montserrat 221, 08041 Barcelona, Spain.
Brain Res. 2004 May 8;1007(1-2):65-70. doi: 10.1016/j.brainres.2004.02.013.
The sigma-1 (sigma(1)) receptors can bind different psychotropic drugs and have been implicated in schizophrenia, depression and dementia. The cloning of the sigma(1)-receptor has allowed to obtain specific antibodies and, in a recent immunohistochemical study, we demonstrated that, in addition to neurons, the sigma(1)-receptor is located in oligodendrocytes [Brain Res. 961 (2003) 92.]. In the present study using in vivo and in vitro techniques, we demonstrate the localization of the sigma(1)-receptor in Schwann cells. Double immunofluorescence studies showed that sigma(1)-receptor co-localized with S100 protein, a specific marker of Schwann cells, in both rat sciatic nerve Schwann cells and Schwann cells in cultures. The sigma(1)-receptor immunoreactivity was seen in the cytoplasm and paranodal region formed by these cells, but not in myelin itself. The presence of sigma(1)-receptor in oligodendrocytes and Schwann cells is discussed on the basis on recent findings involving this receptor in lipid metabolism, compartmentalization and transport to the plasma membrane, thus suggesting a role for sigma(1)-receptor signaling in myelination.
σ1受体可与多种精神药物结合，且与精神分裂症、抑郁症和痴呆症有关。σ1受体的克隆使得获得特异性抗体成为可能，在最近的一项免疫组织化学研究中，我们证实，除神经元外，σ1受体还存在于少突胶质细胞中[《脑研究》961（2003）92]。在本研究中，我们运用体内和体外技术，证实了σ1受体在雪旺细胞中的定位。双重免疫荧光研究表明，在大鼠坐骨神经雪旺细胞和培养的雪旺细胞中，σ1受体均与雪旺细胞的特异性标志物S100蛋白共定位。σ1受体免疫反应性见于这些细胞形成的细胞质和结旁区，但不见于髓鞘本身。基于最近有关该受体在脂质代谢、区室化及向质膜转运方面的研究发现，本文讨论了少突胶质细胞和雪旺细胞中σ1受体的存在情况，这表明σ1受体信号传导在髓鞘形成中发挥作用。
