Schauer R, Haverkamp J, Wember M, Kamerling J P, Vliegenthart J F
Eur J Biochem. 1976 Feb 16;62(2):237-42. doi: 10.1111/j.1432-1033.1976.tb10153.x.
The acylneuraminic acid fraction, obtained on mild acid hydrolysis of glycoproteins from bovine submandibular glands, contains approximately 2% N-acety-9-O-L-lactylneuraminic acid. The compound has been isolated and purified by ion-exchange and cellulose column chromatography. The structure has been elucidated using thin-layer chromatography, colorimetry, gas-liquid chromatography/mass spectrometry, periodate oxidation and specific lactate dehydrogenases. An evaluation of the different analytical methods is given.
通过对牛下颌下腺糖蛋白进行温和酸水解得到的酰基神经氨酸部分，含有约2%的N-乙酰-9-O-L-乳酰神经氨酸。该化合物已通过离子交换和纤维素柱色谱法进行分离和纯化。其结构已通过薄层色谱法、比色法、气-液色谱/质谱法、高碘酸盐氧化法和特定的乳酸脱氢酶进行阐明。并对不同的分析方法进行了评估。
