Nishiura M
Bull Tokyo Med Dent Univ. 1984 Mar;31(1):1-11.
Molecular species of sialic acids released by mild acid hydrolysis from mucins of bovine small and large intestine were characterized by two dimensional thin-layer chromatography and gas chromatography-mass spectrometry. The small and large intestinal mucins contained both N-acetyl- and N-glycolylneuraminic acid. Although O-acetylated derivatives were not detectable in the small intestinal mucin, 9-O-acetyl-N-glycolylneuraminic acid was demonstrated in the large intestinal mucin by thin-layer chromatography and its structure was confirmed by mass spectrometry. Moreover, the presence of three kinds of O-acetyl-N-acetylneuraminic acid and five kinds of O-acetyl-N-glycolylneuraminic acid in the large intestinal mucin was suggested by thin-layer chromatography and mass chromatography. The limited distribution of O-acetylated neuraminic acids suggests that their derivatives are only produced in the mucous glands of the large intestine.
通过二维薄层色谱法和气相色谱 - 质谱联用技术对温和酸水解牛小肠和大肠粘蛋白释放的唾液酸分子种类进行了表征。小肠和大肠粘蛋白均含有N - 乙酰神经氨酸和N - 羟乙酰神经氨酸。虽然在小肠粘蛋白中未检测到O - 乙酰化衍生物，但通过薄层色谱法在大肠粘蛋白中证实了9 - O - 乙酰 - N - 羟乙酰神经氨酸的存在，并通过质谱法确定了其结构。此外，薄层色谱法和质谱色谱法表明大肠粘蛋白中存在三种O - 乙酰 - N - 乙酰神经氨酸和五种O - 乙酰 - N - 羟乙酰神经氨酸。O - 乙酰化神经氨酸的有限分布表明其衍生物仅在大肠的粘液腺中产生。
