[The expression and mechanism study of BMP in pleomorphic adenoma].

OBJECTIVE

One important pathological character of pleomorphic adenomas is that there is chorndriod component in the epithelial tumors. It is well known that bone morphogenetic protein (BMPs) plays important roles in the morphogenesis of hard tissue by inducing mesenchymal cells to differentiate into osteoblasts and chondroblasts in vivo. So we examined the expression of BMP and BMP2 mRNA in pleomorphic adenomas to study the pathological mechanism of chorndriod tissue formation.

METHODS

Using ABC immunohistochemistry method, 6 specimens of pleomorphic adenomas were examined with BMP antibody. Reverse transcriptase-polymerase chain reaction (RT-PCR) methods were developed for detecting BMP2 mRNA expression with special primers used to amplify the BMP2 cDNA mature domain fragment. The total RNA was extracted from frozen 6 specimens of pleomorphic adenoma and then reversed to cDNA by reverse transcription using AMV reverse transcriptase. PCR conditions were 94 degrees C for 20 seconds, 60 degrees C for 30 seconds, and 72 degrees C for 1 minute, 40 cycles, followed by extension for 30 minute at 72 degrees C. The reaction products were analyzed by electrophoresis.

RESULTS

BMP expression could be found in all of the specimens of pleomorphic adenoma examined by immunohistochemistry method. BMP2 RT-PCR product was detected in only 5 specimens that showed BMP2 mRNA existed in 5 specimens.

CONCLUSION

These results may be helpful to study the mechanism of pleomorphic adenoma. It is speculated that BMP secreted by tumor cells may play important roles in origin of osteoid tissue in pleomorphic adenoma by inducing neoplastic myoepithelial cells to differentiate to chondrocytes.