Lian K, Du J, Rao Z, Luo H
Department of Orthopedics, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022.
J Tongji Med Univ. 2001;21(4):304-7. doi: 10.1007/BF02886563.
To investigate the effect of calcitonin gene-related peptide (CGRP) on bone resorption mediated by interleukin-1 beta(IL-1 beta) in vitro, the osteoclasts isolated from the long bones of newborn SD rats were co-cultured with osteoblasts on ivory slices placed in 24-well plates. 24 h later, conditioned media containing CGRP and/or IL-1 beta were added to the wells respectively, and continued culturing for 48 h. After the cells were stripped off by ultrasonication, the ivory slices were stained in toludine blue. The number and the total area of resorption lacunae on each slice were measured by computer imaging analysis system. Our results showed that IL-1 beta significantly stimulated bone resorption, but CGRP inhibited the effect mediated by IL-1 beta in a dose-dependent manner. It is suggested that CGRP may inhibit osteoclastic bone resorption through two ways: One is that CGRP functions directly on osteoclasts to block their activation; the other is that CGRP regulates the release of cytokines by osteoblasts and indirectly affects the function of osteoclasts.
为了在体外研究降钙素基因相关肽（CGRP）对白细胞介素-1β（IL-1β）介导的骨吸收的影响，将从新生SD大鼠长骨分离的破骨细胞与成骨细胞在置于24孔板中的象牙薄片上共培养。24小时后，分别向孔中加入含有CGRP和/或IL-1β的条件培养基，并继续培养48小时。通过超声处理使细胞脱落后，将象牙薄片用甲苯胺蓝染色。用计算机图像分析系统测量每片上吸收陷窝的数量和总面积。我们的结果表明，IL-1β显著刺激骨吸收，但CGRP以剂量依赖的方式抑制IL-1β介导的作用。提示CGRP可能通过两种方式抑制破骨细胞性骨吸收：一是CGRP直接作用于破骨细胞以阻断其活化；二是CGRP调节成骨细胞释放细胞因子并间接影响破骨细胞的功能。
