Sun X, Zou W, Zhao C
Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Teconology, Wuhan 430030.
J Tongji Med Univ. 2001;21(3):263-4. doi: 10.1007/BF02886449.
The apoptosis of lens epithelial cells (LECs) induced by ultraviolet and the expression of P53 were investigated. Wistar rats received 100 mW/m2 ultraviolet irradiation (UVR) (lambda = 280 nm-315 nm) for 15 min. One, 6, 24 h after irradiation the lens capsules were dissected. The percentages of apoptotic cells were evaluated by the TdT-dUTP terminal nick-end labeling (TUNEL) technique and the expression of P53 was detected by using immunohistochemical assay. The results showed that the percentages of TUNEL-positive nuclei at 24 h after irradiation was significantly higher than in the control group and those 1 h, 6 h after irradiation. The percentages of P53-positive cells at 6 h, 24 h after irradiation were significantly higher than in the control group and those 1 h after irradiation. It was concluded that UVR could induce the apoptosis of lens epithelial cell. The expression of P53 might be responsible for the apoptosis of lens epithelial cells.
研究了紫外线诱导的晶状体上皮细胞（LECs）凋亡及P53的表达。将Wistar大鼠置于100 mW/m2紫外线照射（UVR）（波长=280 nm - 315 nm）下15分钟。照射后1、6、24小时解剖晶状体囊。采用TdT - dUTP末端脱氧核苷酸转移酶介导的缺口末端标记（TUNEL）技术评估凋亡细胞的百分比，并使用免疫组织化学测定法检测P53的表达。结果显示，照射后24小时TUNEL阳性细胞核的百分比显著高于对照组以及照射后1小时、6小时的水平。照射后6小时、24小时P53阳性细胞的百分比显著高于对照组以及照射后1小时的水平。结论是，UVR可诱导晶状体上皮细胞凋亡。P53的表达可能是晶状体上皮细胞凋亡的原因。
