Vienna Institute for Research in Ocular Surgery, Hanusch Hospital, Vienna, Austria.
Ludwig Boltzmann Institute of Osteology at Hanusch Hospital of OEGK and AUVA Trauma Centre Meidling, 1st Medical Department, Hanusch Hospital, Vienna, Austria.
Acta Ophthalmol. 2021 Jun;99(4):e587-e593. doi: 10.1111/aos.14641. Epub 2020 Oct 30.
The aim of the present study was to determine whether caffeine concentrations in human lens epithelial cells (LECs) achieved from acute peroral caffeine intake inhibit ultraviolet radiation-induced apoptosis in vitro.
Patients were planned for cataract surgery of both eyes with a caffeine abstinence of 2 weeks in total, starting 1 week before surgery of the first eye. The second eye was scheduled 1 week after the first eye. At the day of the second eye surgery, patients were given coffee containing 180 mg caffeine shortly before surgery. Lens capsules including LEC, harvested after capsulorhexis, were transferred to a cell culture dish and immediately exposed to close to threshold ultraviolet radiation (UVR). At 24 hr after UVR exposure, apoptotic LECs were analysed by TdT-mediated dUTP-biotin nick end labeling (TUNEL) staining.
TUNEL-positive cells were detected in UVR-exposed lens capsules both after caffeine intake and in controls. The mean difference in TUNEL-positive cells between caffeine intake and contralateral controls (no caffeine) resulted in a 95% CI 15.3 ± 10.4% (degrees of freedom: 16).
Peroral caffeine consumption significantly decreased UVR-induced apoptosis in LEC supporting epidemiological findings that caffeine delays the onset of cataract.
本研究旨在确定从急性口服咖啡因摄入中获得的人晶状体上皮细胞 (LEC) 中的咖啡因浓度是否能抑制体外紫外线辐射诱导的细胞凋亡。
计划让患者双眼均接受白内障手术，总禁食咖啡因时间为 2 周，从第一只眼手术前 1 周开始。第二只眼计划在第一只眼手术后 1 周进行。在第二只眼手术当天，患者在手术前短时间内饮用含有 180mg 咖啡因的咖啡。晶状体囊包括晶状体囊切开术后收获的 LEC，转移到细胞培养皿中，并立即暴露于接近阈值的紫外线辐射 (UVR) 下。在 UVR 暴露后 24 小时，通过末端转移酶介导的 dUTP-生物素缺口末端标记 (TUNEL) 染色分析凋亡的 LEC。
在接受 UVR 照射的晶状体囊中，无论是在摄入咖啡因后还是在对照组中，都检测到 TUNEL 阳性细胞。咖啡因摄入和对侧对照组（无咖啡因）之间 TUNEL 阳性细胞的平均差异导致 95%CI 为 15.3±10.4%（自由度：16）。
口服咖啡因摄入可显著减少 LEC 中的 UVR 诱导的细胞凋亡，支持咖啡因可延迟白内障发病的流行病学发现。
