Yang J, Fang Z, Li H, Zhang C
Department of Stomatology, Southwest Hospital, Third Military Medical University.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2001 Dec;19(6):360-2.
The aim of this study is to investigate the relationship between the expression changes of bcl-2 and Bax genes and their functions in facial neuronal apoptosis after amputating the facial nerve.
Rats were randomized into two groups, including the experimental and the control group. The changes of expression of bcl-2 and Bax genes in the facial neurons and facial neuronal apoptosis were observed after the facial nerve amputation using the methods of ABC immunohistochemical staining and TUNEL.
The expression of bcl-2 and Bax genes and the apoptosis of facial neurons were significantly higher in the experimental group than those of the control group from the 3rd to 21st day after amputation, but the bcl-2/Bax significantly decreased compared with that of the control group.
This indicated that bcl-2 and Bax might play an important regulation role in the process of facial neurons apoptosis following facial nerve amputation.
本研究旨在探讨面神经切断术后bcl-2和Bax基因表达变化与其在面神经细胞凋亡中的作用之间的关系。
将大鼠随机分为两组，即实验组和对照组。采用ABC免疫组织化学染色和TUNEL法观察面神经切断术后面神经细胞中bcl-2和Bax基因表达变化及面神经细胞凋亡情况。
术后第3天至第21天，实验组bcl-2和Bax基因表达及面神经细胞凋亡均明显高于对照组，但bcl-2/Bax比值明显低于对照组。
这表明bcl-2和Bax可能在面神经切断术后面神经细胞凋亡过程中起重要调节作用。
