Ponder Elizabeth L, Fried Bernard, Sherma Joseph
Department of Chemistry, Lafayette College, Easton, PA 18042, USA.
Parasitol Res. 2003 Feb;89(3):242-4. doi: 10.1007/s00436-002-0760-y. Epub 2002 Nov 6.
Thin layer chromatography (TLC) was used to analyze the amino acids in worm incubates isotonic and hypotonic to the intestinal habitat of adult Echinostoma caproni and to analyze the free pool amino acids of these trematodes after incubation. Qualitative analysis revealed the presence of histidine, lysine, alanine, and proline in all samples of incubate and worm tissue. Quantification of histidine and lysine by TLC with densitometry gave mean concentrations of 24.1 micro g histidine/g worm per ml incubate in Locke's solution and 195.0 micro g lysine/g worm per ml incubate in deioinized (DI) water. Quantification of histidine and lysine in the worm tissue gave mean weight percents of 0.0587 and 0.0263, respectively, in worms incubated in Locke's solution and 0.0229 and 0.0163, respectively, for worms incubated in DI water. Our findings suggest that E. caproni adults may leak amino acids for osmoregulation in hypotonic environments.
采用薄层色谱法（TLC）分析了与卡氏棘口吸虫成虫肠道栖息地等渗和低渗的蠕虫培养液中的氨基酸，并分析了培养后这些吸虫的游离氨基酸库。定性分析显示，在所有培养液和蠕虫组织样本中均存在组氨酸、赖氨酸、丙氨酸和脯氨酸。通过薄层色谱法和密度测定法对组氨酸和赖氨酸进行定量分析，结果表明，在洛克溶液中，每毫升培养液中组氨酸的平均浓度为24.1微克/克蠕虫，在去离子水（DI）中，每毫升培养液中赖氨酸的平均浓度为195.0微克/克蠕虫。对蠕虫组织中的组氨酸和赖氨酸进行定量分析，结果表明，在洛克溶液中培养的蠕虫中，组氨酸和赖氨酸的平均重量百分比分别为0.0587和0.0263，在去离子水中培养的蠕虫中，组氨酸和赖氨酸的平均重量百分比分别为0.0229和0.0163。我们的研究结果表明，卡氏棘口吸虫成虫可能在低渗环境中为了渗透调节而泄漏氨基酸。
