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[从豚鼠耳蜗不同旋中分离外毛细胞]

[Isolation of outer hair cells from varying turns of the guinea-pig cochlea].

作者信息

Guo Y, Su Z, Yang W, Jiang S

机构信息

Institute of Otolaryngology, Chinese PLA General Hospital, Beijing 100853.

出版信息

Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2001 Jan;15(1):26-7.

Abstract

OBJECTIVE

To probe the method of isolating outer hair cells (OHC) from each of four turns of the guinea-pig cochlea.

METHOD

From eight guinea pigs the organ of Corti from each of four turns of the cochlea were dissected, and then treated using enzyme.

RESULT

A fair amount of living OHCs from each of four turns were obtained. The length of OHCs from each of four turns were 23.81, 34.50, 60.48 and 71.37 microns.

CONCLUSION

The key to success in isolating OHCs from each of four turns of the cochlea is to know very well the anatomical characteristics of each of four turns of the cochlea and be operated in accordance with normal rules.

摘要

目的

探索从豚鼠耳蜗四个转中分别分离外毛细胞(OHC)的方法。

方法

从八只豚鼠身上解剖出耳蜗四个转中的每个转的柯蒂氏器,然后进行酶处理。

结果

从四个转中均获得了相当数量的活外毛细胞。四个转中每个转的外毛细胞长度分别为23.81、34.50、60.48和71.37微米。

结论

从耳蜗四个转中成功分离外毛细胞的关键在于熟知耳蜗四个转各自的解剖特征并按常规操作。

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