Tian Yuan, Yu Li
Department of Physiology, Fundamental School of LiaoNing Medical College, Jinzhou 121001, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2008 Aug;24(3):373-6.
The comparison of several methods which were used to isolate cochlear outer hair cells and the observations of morphology were researched.
Three different separating methods of outer hair cells in cochlea were adopted; the morphology of outer hair cells in cochlea and the morphology of cochlear stretched preparation in Silver Nitrate staining were also investigated.
Single alive OHC in cochlea was disassociated by all methods, with microscope and cochlear stretched preparation's staining. We could also observe appearances and distributions of OHC in cochlea.
It is successful to isolate single alive cochlear OHC, and it will be very important to investigate deeply normal physiological functions and changes of functions and morphology in some pathologic status in cochlear OHCs.
比较几种用于分离耳蜗外毛细胞的方法并观察其形态。
采用三种不同的耳蜗外毛细胞分离方法；同时研究耳蜗外毛细胞的形态以及硝酸银染色的耳蜗铺片的形态。
所有方法均能分离出耳蜗中单个活的外毛细胞，并可通过显微镜及耳蜗铺片染色观察到耳蜗中外毛细胞的外观及分布。
成功分离出单个活的耳蜗外毛细胞，这对于深入研究耳蜗外毛细胞的正常生理功能以及某些病理状态下其功能和形态的变化具有重要意义。
