Wei Gui-Lin, Liu Xue-Liang, Li Jing-Hua, Liu Ying, Shang Zhen-Hua
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116012, China.
Se Pu. 2002 Mar;20(2):108-14.
Three kinds of affinity membranes utilized for endotoxin removal, namely, chitosan affinity membrane (KFCC517), hydrophobic and cationic charged membrane (KFCG316) and metal chelate affinity membrane (KFCM402) were prepared by using cellulose membrane as support. The adsorbability and applications of the membranes in endotoxin removal were investigated. The results showed that all of these affinity membranes could be utilized for endotoxin removal in several kinds of solutions. The factors affecting endotoxin removal such as ionic strength, pH value and flow rate were investigated, and the results were discussed. Three cartridges of membranes KFCC517, KFCG316 and KFCM402 were utilized for endotoxin removal in human serum albumin solution. The cartridge of membrane KFCC517 also was utilized in medicine injections, such as hydrocortisone, puerarin, tetracaine hydrochloride and dextran 40 glucose. Under the optimal conditions, the removal efficiencies of endotoxin were over 80% and the recoveries of desirable substances were over 95%.
以纤维素膜为载体，制备了三种用于去除内毒素的亲和膜，即壳聚糖亲和膜（KFCC517）、疏水阳离子荷电膜（KFCG316）和金属螯合亲和膜（KFCM402）。研究了这些膜对内毒素的吸附性能及其在去除内毒素方面的应用。结果表明，所有这些亲和膜均可用于去除多种溶液中的内毒素。考察了离子强度、pH值和流速等影响内毒素去除的因素，并对结果进行了讨论。使用KFCC517、KFCG316和KFCM402三种膜柱去除人血清白蛋白溶液中的内毒素。KFCC517膜柱还用于氢化可的松、葛根素、盐酸丁卡因和右旋糖酐40葡萄糖等药物注射剂中。在最佳条件下，内毒素去除率超过80%，目标物质回收率超过95%。
