在以除草剂2,2-二氯丙酸（茅草枯）进行富集和筛选后，从原始土壤中分离出的细菌中α-卤代羧酸脱卤酶的多样性。

Diversity of alpha-halocarboxylic acid dehalogenases in bacteria isolated from a pristine soil after enrichment and selection on the herbicide 2,2-dichloropropionic acid (Dalapon).

作者信息

Marchesi Julian R, Weightman Andrew J

机构信息

Cardiff School of Biosciences, Main Building, Cardiff University, PO Box 915, UK.

出版信息

Environ Microbiol. 2003 Jan;5(1):48-54. doi: 10.1046/j.1462-2920.2003.00384.x.

DOI:10.1046/j.1462-2920.2003.00384.x

PMID:12542712

Abstract

Five pure cultures of bacteria (strains DA1-5) able to degrade 2,2-dichloropropionic acid (22DCPA) were isolated for the first time from pristine bulk soil samples. From 16S rDNA analysis, it was concluded that strains DA2, DA3 and DA4 were members of the Bradyrhizobium subgroup (alpha-Proteobacteria), strain DA5 clustered in the Brucella assemblage (alpha-Proteobacteria) and strain DA1 clustered in the beta-Proteobacteria. Biochemical and molecular analysis of the dehalogenases from the isolates showed that these enzymes were quite diverse. Several dehalogenases were closely related to group I and II alpha-halocarboxylic acid dehalogenases, and partial polymerase chain reaction (PCR) products were obtained from isolates DA1, 2, 3 and 4 using degenerate dehalogenase primers. However, no PCR products were obtained from isolate DA5 using either of the group I or II alpha-halocarboxylic acid dehalogenase primers. Isolates DA2 and DA4 contained putative silent dehalogenases. The investigation highlighted the endemic nature of these genes in pristine environments and how diverse these were even from spatially close samples.

摘要

首次从原始土壤样本中分离出5种能够降解2,2-二氯丙酸（22DCPA）的纯细菌培养物（菌株DA1-5）。通过16S rDNA分析得出，菌株DA2、DA3和DA4属于慢生根瘤菌亚群（α-变形菌纲），菌株DA5聚集在布鲁氏菌属组合（α-变形菌纲）中，而菌株DA1聚集在β-变形菌纲中。对分离菌株的脱卤酶进行生化和分子分析表明，这些酶差异很大。几种脱卤酶与I组和II组α-卤代羧酸脱卤酶密切相关，使用简并脱卤酶引物从菌株DA1、2、3和4中获得了部分聚合酶链反应（PCR）产物。然而，使用I组或II组α-卤代羧酸脱卤酶引物均未从菌株DA5中获得PCR产物。菌株DA2和DA4含有推定的沉默脱卤酶。该研究突出了这些基因在原始环境中的特有性质，以及即使来自空间距离很近的样本，它们也具有很大的多样性。