Graff Andrea, Stubner Stephan
Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.
Syst Appl Microbiol. 2003 Sep;26(3):445-52. doi: 10.1078/072320203322497482.
In rice paddy soils an active cycling of sulfur compounds takes place. To elucidate the diversity of thiosulfate-oxidizing bacteria these organisms were enriched from bulk soil and rice roots by the most probable number method in liquid medium. From the MPN enrichment cultures 21 bacterial strains were isolated on solid mineral medium, and could be further shown to produce sulfate from thiosulfate. These strains were characterized by 16S rDNA analyses. The isolates were affiliated to seven different phylogenetic groups within the alpha- and beta-subclass of Proteobacteria. Two of these phylotypes were already described as S-oxidizers in this environment (Xanthobacter sp. and Bosea sp. related strains), but five groups represented new S-oxidizers in rice field soil. These isolates were closely related to Mesorhizobium loti, to Hydrogenophaga sp., to Delftia sp., to Pandoraea sp. or showed sequence similarity to a strain of Achromobacter sp.
在稻田土壤中，硫化合物存在活跃的循环。为了阐明硫代硫酸盐氧化细菌的多样性，通过液体培养基中的最大可能数法从大量土壤和水稻根系中富集这些微生物。从MPN富集培养物中，在固体矿物培养基上分离出21株细菌菌株，并且进一步证明它们能够从硫代硫酸盐产生硫酸盐。通过16S rDNA分析对这些菌株进行了表征。这些分离株隶属于变形菌纲α-和β-亚类中的七个不同系统发育组。其中两个系统型在这种环境中已被描述为硫氧化剂（与黄色杆菌属和博斯氏菌属相关的菌株），但有五个组代表了稻田土壤中的新型硫氧化剂。这些分离株与百脉根中生根瘤菌、嗜氢菌属、代尔夫特菌属、潘多拉菌属密切相关，或者与无色杆菌属的一个菌株表现出序列相似性。
