Pouliot Frédéric, Blais Alexandre, Labrie Claude
Oncology and Molecular Endocrinology Research Center, Centre Hospitalier de l'Université Loval Research Center Centre Hospitalier Universitaire de Quebec and Laval University, Sainte-Foy, Quebec, G1V 4G2 Canada.
Cancer Res. 2003 Jan 15;63(2):277-81.
Bone morphogenetic proteins (BMPs) exert cell type-specific effects on cell proliferation. To clarify the role of the BMP pathway in human breast cancer cells, we used a dominant negative strategy with a truncated human type II BMP receptor (DN-BMPRII; amino acid 1-172) fused to the NH2 terminus of enhanced green fluorescent protein. Transient overexpression of DN-BMPRII interfered with BMP-2-induced Smad1 transcriptional activity and caused cells to accumulate in G1. Stable cell lines that constitutively overexpressed DN-BMPRII were resistant to BMP-2-induced Smad1 phosphorylation and proliferated much more slowly than control stable cell lines. These results suggest that BMPs interacting with type II BMP receptors contribute to the proliferation and/or survival of human breast cancer cells.
骨形态发生蛋白（BMPs）对细胞增殖具有细胞类型特异性作用。为阐明BMP信号通路在人乳腺癌细胞中的作用，我们采用了一种显性负性策略，即将截短的人II型BMP受体（DN-BMPRII；氨基酸1-172）与增强型绿色荧光蛋白的NH2末端融合。DN-BMPRII的瞬时过表达干扰了BMP-2诱导的Smad1转录活性，并导致细胞在G1期积累。持续过表达DN-BMPRII的稳定细胞系对BMP-2诱导的Smad1磷酸化具有抗性，且增殖速度比对照稳定细胞系慢得多。这些结果表明，与II型BMP受体相互作用的BMPs有助于人乳腺癌细胞的增殖和/或存活。
