宽口径不锈钢填充毛细管液相色谱柱的制备与评价
[Preparation and evaluation of wide-bore stainless-steel packed capillary liquid chromatographic columns].
作者信息
Tang Y H, Zhu D Q, Guan Y F
机构信息
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116012, China.
出版信息
Se Pu. 2001 Jul;19(4):289-92.
A zero-dead volume column end and a conical shaped slurry reservoir for wide-bore stainless-steel packed capillary liquid chromatographic columns were designed and evaluated. A detailed procedure for the preparation of reversed-phase stainless-steel packed capillary columns with 0.5 mm-1.0 mm i.d. is described. The influences of the column length and the packing material on the column performance have been studied. Columns were evaluated by the reduced plate height vs reduced velocity curve and the peak asymmetric factor. Experimental results showed that the column efficiency and the reproducibility were better than 75% of theoretical value and 6% RSD, respectively. Separations of antiepileptic drugs and chlorinated benzenes are demonstrated.
设计并评估了一种用于宽内径不锈钢填充毛细管液相色谱柱的零死体积柱端和锥形浆料储存器。描述了制备内径为0.5 mm - 1.0 mm的反相不锈钢填充毛细管柱的详细步骤。研究了柱长和填充材料对柱性能的影响。通过折合板高与折合流速曲线和峰不对称因子对色谱柱进行评估。实验结果表明，柱效和重现性分别优于理论值的75%和6%的相对标准偏差。展示了抗癫痫药物和氯苯的分离。
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