用于制备液相色谱的开口角度为10度的锥形柱研究。
Study of conical columns with 10 degrees opening angle for preparative liquid chromatography.
作者信息
Guan Wenna, Xu Feng, Tan Feng, Guan Yafeng
机构信息
Department of Analytical Chemistry and Micro-Instrumentation, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116012, China.
出版信息
J Chromatogr A. 2006 Mar 10;1108(2):218-24. doi: 10.1016/j.chroma.2006.01.042. Epub 2006 Feb 7.
The efficiency and sample capacity of conical liquid chromatographic columns with 10 degrees opening angle were studied at different ratio of cross section areas of inlet to outlet (A(in)/A(out)) and column dimension. As the A(in)/A(out) ratio changed from 4 to 2.25, the reduced plate height (h) was reduced and the h value decreased 12% when the column dimension was scaled up proportionally because of relatively smaller dead volume on both end of the column. Compared to cylindrical columns having corresponding lengths and volumes, the conical columns with 10 degrees opening angle were superior in column efficiency, resolution and the maximum peak concentration at column outlet; the loadability of conical column was improved 30-40% on injection volume and 50-60% on sample mass, respectively, over cylindrical columns.
研究了开口角度为10度的锥形液相色谱柱在不同进样口与出口截面积比（A(in)/A(out)）和柱尺寸下的效率和样品容量。当A(in)/A(out)比从4变为2.25时，折合板高（h）降低，并且当柱尺寸按比例放大时，由于柱两端的死体积相对较小，h值降低了12%。与具有相应长度和体积的圆柱形柱相比，开口角度为10度的锥形柱在柱效率、分离度和柱出口处的最大峰浓度方面更具优势；锥形柱的进样量负载能力比圆柱形柱分别提高了30 - 40%，样品质量负载能力提高了50 - 60%。
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