Zhang G G, Zeng H, Xu S X
Shenyang Pharmaceutical University, Shenyang 110015, China.
Se Pu. 2001 Jul;19(4):365-6.
Icariin in "Tangzhi Shuangjiang Cha" was quantitatively determined by HPLC with ODS column, a mixture of MeOH-H2O(60:40, V/V) as mobile phase and UV detection at 270 nm. There was a good linear relationship within the range of 0.1 g/L-0.5 g/L(r = 0.9993). The average recovery was 101.4%, RSD was below 2.7%(n = 3). The method is rapid, simple, accurate and reproducible. And the method is proposed to be used for quality control of "Tangzhi Shuangjiang Cha".
采用高效液相色谱法，以ODS柱，甲醇-水（60:40，V/V）混合液为流动相，在270nm波长处进行紫外检测，对“糖脂双降茶”中的淫羊藿苷进行定量测定。在0.1g/L - 0.5g/L范围内线性关系良好（r = 0.9993）。平均回收率为101.4%，相对标准偏差低于2.7%（n = 3）。该方法快速、简便、准确、重现性好。建议该方法用于“糖脂双降茶”的质量控制。
