Chen Xianggui, Wang Ruishu, Deng Maoxian, Ran Xinze
Department of Food Engineering, Sichuan University of Science and Technology, Chengdu 610039, China.
Wei Sheng Yan Jiu. 2002 Jun;31(3):168-71.
The effect of exogenous nucleotides on proliferation and migration of a normal rat small intestinal epithelial cell line IEC-6 is studied. The concentration-effects as well as interaction of exogenous nucleotides on proliferation of IEC-6 are measured by MTT. Migration of IEC-6 after wounded is determined by an in vitro model of intestinal epithelial restitution of IEC-6 monolayer. Expression of TGF beta is detected by immunohistochemistry. The results show AMP and GMP remarkably inhibit proliferation of IEC-6 in concentration-dependent manner respectively at 30 mumol/L or 150 mumol/L and more. CMP, UMP and nucleotides mixture can not enhance or inhibit the growth with the exception of inhibition of CMP on proliferation at very high concentration (1440 mumol/L). In contrast, CMP, especially UMP, can remarkably abolish the proliferation-inhibiting effects of AMP or GMP on the cell, when AMP or GMP is supplemented. Nucleotides mixture significantly facilitate migration of IEC-6 after wounded but fail to promote the expression of TGF beta. It is concluded that purine nucleotides inhibits proliferation of IEC-6. Pyrimidine nucleotides can abolish the inhibitive effects of purine nucleotides, and Nucleotides mixture promotes migration of IEC-6 after wounded by a TGF beta-independented way.
研究外源性核苷酸对正常大鼠小肠上皮细胞系IEC-6增殖和迁移的影响。采用MTT法检测外源性核苷酸对IEC-6增殖的浓度效应及相互作用。通过IEC-6单层肠上皮修复的体外模型测定损伤后IEC-6的迁移。采用免疫组织化学法检测转化生长因子β（TGFβ）的表达。结果显示，AMP和GMP分别在30μmol/L或150μmol/L及以上时，以浓度依赖性方式显著抑制IEC-6的增殖。CMP、UMP和核苷酸混合物除在极高浓度（1440μmol/L）时CMP抑制增殖外，均不能增强或抑制生长。相反，当添加AMP或GMP时，CMP尤其是UMP可显著消除AMP或GMP对细胞的增殖抑制作用。核苷酸混合物显著促进损伤后IEC-6的迁移，但未能促进TGFβ的表达。结论是嘌呤核苷酸抑制IEC-6的增殖。嘧啶核苷酸可消除嘌呤核苷酸的抑制作用，核苷酸混合物通过非TGFβ依赖的方式促进损伤后IEC-6的迁移。
