Chen Xiang-gui, Wang Rui-shu, Deng Mao-xian, Ran Xin-ze
School of Bioengineering, Xihua University, Chengdu 610039, China.
Wei Sheng Yan Jiu. 2005 Nov;34(6):701-4.
To investigate the effects of exogenous nucleotides on apoptosis of a normal rat small intestinal epithelial cell line, IEC-6.
Cultured IEC-6 cells were treated by four kinds of monophosphate nucleotides and their mixture prepared according to their composition in human milk, then the cell apoptosis was determined by flow cytometry measurement, morphologic characterization, and electron-microscope observation.
IEC-6 cells treated with AMP or GMP showed a apotosis peak in flow cytometry measurement, but only AMP produce typical apoptosis characteristics in electron-microscope observation. Pyrimidine nucleotides (UMP and CMP)and nucleotides mixture could not induce apoptosis. However, UMP could significantly eliminate the apoptosis-inducing effects of AMP or GMP.
Purine nucleotides induce apoptosis of IEC-6, inducing effects of purine nucleotides. pyrimidine nucleotides UMP could abolish the apoptosis-inducing effects of purine nucleotides.
研究外源性核苷酸对正常大鼠小肠上皮细胞系IEC-6凋亡的影响。
用四种单磷酸核苷酸及其按人乳中成分配制的混合物处理培养的IEC-6细胞，然后通过流式细胞术检测、形态学特征观察和电子显微镜观察来测定细胞凋亡情况。
用AMP或GMP处理的IEC-6细胞在流式细胞术检测中出现凋亡峰，但只有AMP在电子显微镜观察中呈现典型的凋亡特征。嘧啶核苷酸（UMP和CMP）以及核苷酸混合物不能诱导凋亡。然而，UMP能显著消除AMP或GMP的凋亡诱导作用。
嘌呤核苷酸诱导IEC-6凋亡，嘌呤核苷酸有诱导作用。嘧啶核苷酸UMP能消除嘌呤核苷酸的凋亡诱导作用。
