Sun Wenguang, Sun Changhao, Wang Shuran, Fu Rongxia
School of Public Health, Harbin Medical University, Harbin 150001, China.
Wei Sheng Yan Jiu. 2002 Jun;31(3):174-7.
In order to study the effect of iron on the expression of UCP2 in white fat and UCP3 in muscle in obese rats, a reverse transcription polymerase chain reaction (RT-PCR) technique is used to measure the expression of UCP2 and UCP3 mRNA. The results show: (1) Adequate iron improves the level of thyroid hormone in blood serum; (2) the body fat content of obese rat in 5, 10, and 20 times of iron supplement groups is lower than that of obese rats in normal iron supplement group; (3) the UCP2 and UCP3 mRNA expression of iron-deficient obese rats is low in iron-deficient obese rats; (4) iron supplement enhances the level of UCP3 mRNA expression in muscle and the level of UCP3 mRNA is the highest in the 5 times iron supplement group and about 2 times of that of basic diet group. However, there is no effect on the UCP2 mRNA expression induced by high energy diet. It suggested that adequate iron can improve the level of serum thyroid hormone, induce the UCP3 mRNA expression in muscle and mobilize fat to produce heat, but had no effect on UCP2 mRNA in white fat of obese rat.
为研究铁对肥胖大鼠白色脂肪组织中解偶联蛋白2（UCP2）及肌肉中解偶联蛋白3（UCP3）表达的影响，采用逆转录聚合酶链反应（RT-PCR）技术检测UCP2和UCP3 mRNA的表达。结果显示：（1）充足的铁可提高血清甲状腺激素水平；（2）铁补充量为正常量5倍、10倍和20倍的肥胖大鼠组体脂含量低于正常铁补充量的肥胖大鼠组；（3）缺铁肥胖大鼠UCP2和UCP3 mRNA表达较低；（4）补充铁可提高肌肉中UCP3 mRNA表达水平，其中铁补充量为5倍组UCP3 mRNA水平最高，约为基础饮食组的2倍。但对高能饮食诱导的UCP2 mRNA表达无影响。提示充足的铁可提高血清甲状腺激素水平，诱导肌肉中UCP3 mRNA表达，动员脂肪产热，但对肥胖大鼠白色脂肪组织中UCP2 mRNA无影响。
