Yu Xinfeng, Sun Changhao, Liu Rong, Li Ying
Department of Nutrition and Food Hygiene, Harbin Medical University, Harbin 150001, China.
Wei Sheng Yan Jiu. 2003 May;32(3):204-7.
In order to study the effects of dietary calcium on the expression of uncoupling protein 3(UCP3) in skeletal muscle of Wistar rat fed with high fat diet, 54 male Wistar rats were randomly divided into 6 groups: Group A was fed with basic diet(0.5% calcium), Group B was fed with high fat low calcium diet(0.08% calcium), Group C was fed with high fat normal calcium diet(0.5% calcium), Group D and E was fed with high fat high calcium diet(1% and 1.5% calcium respectively), Group F was fed with high fat high calcium diet (1.5% calcium) supplemented with vitamin D(600 IU%). Reverse transcription polymerase chain reaction (RT-PCR) was used to measure the level of UCP3 mRNA. The results showed that the body weight and fat content in group C were significantly higher than those in group A. However, the body weight gain in groups of D, E, and F was less than those in group C. The serum insulin in group C increased significantly compared with that in groups of A, D, E and F(P < 0.05), but there was no significant difference in blood glucose among all groups. Compared with group A, the level of serum leptin increased significantly in group C(P < 0.05) and increased slightly in groups D, E and F (P > 0.05). The level of serum FT3 in groups D, E and F increased significantly compared with that of group A, B and C(P < 0.05), but there was no significant difference in FT4 among all groups(P > 0.05). Compared with group A, the expression of UCP3 mRNA decreased in skeletal muscle in group C, while UCP3 mRNA increased in groups D, E and F compared with that of group C. In conclusion, dietary calcium may not only reduce the body weight gain induced by high fat diet, and improve hyperinsulinemia, but also may enhance the expression of UCP3 mRNA in skeletal muscle by increasing the serum level of leptin and FT3 which may play the role in the prevention of obesity.
为研究膳食钙对高脂饮食喂养的Wistar大鼠骨骼肌解偶联蛋白3（UCP3）表达的影响，将54只雄性Wistar大鼠随机分为6组：A组喂基础饲料（钙含量0.5%），B组喂高脂低钙饲料（钙含量0.08%），C组喂高脂正常钙饲料（钙含量0.5%），D组和E组喂高脂高钙饲料（钙含量分别为1%和1.5%），F组喂高脂高钙饲料（钙含量1.5%）并补充维生素D（600 IU%）。采用逆转录聚合酶链反应（RT-PCR）检测UCP3 mRNA水平。结果显示，C组大鼠体重和脂肪含量显著高于A组。然而，D、E、F组大鼠体重增加量低于C组。与A、D、E、F组相比，C组大鼠血清胰岛素显著升高（P < 0.05），但各组血糖无显著差异。与A组相比，C组大鼠血清瘦素水平显著升高（P < 0.05），D、E、F组略有升高（P > 0.05）。与A、B、C组相比，D、E、F组大鼠血清FT3水平显著升高（P < 0.05），各组FT4无显著差异（P > 0.05）。与A组相比，C组大鼠骨骼肌UCP3 mRNA表达降低，而与C组相比，D、E、F组UCP3 mRNA表达升高。综上所述，膳食钙不仅可能降低高脂饮食诱导的体重增加，改善高胰岛素血症，还可能通过提高血清瘦素和FT3水平增强骨骼肌UCP3 mRNA表达，这可能在预防肥胖中发挥作用。
