在小鼠神经肌肉接头处进行¹²⁵I-α-银环蛇毒素结合后，通过电子显微镜放射自显影法定量测定接头处和接头外乙酰胆碱受体。
Quantitation of junctional and extrajunctional acetylcholine receptors by electron microscope autoradiography after 125I-alpha-bungarotoxin binding at mouse neuromuscular junctions.
作者信息
Fertuck H C, Salpeter M M
出版信息
J Cell Biol. 1976 Apr;69(1):144-58. doi: 10.1083/jcb.69.1.144.
Abstract
The distribution and quantitation of 125I-alpha-bungarotoxin (alpha-BTX) binding sites and thus acetylcholine receptor (AChR) were determined in mouse sternomastoid muscle by electron microscope autoradiography. We found that a valid criterion for receptor saturation at the neuromuscular junction was the complete elimination of neurally evoked tetanic muscle contractions, since, when such a criterion was used for the endpoint of toxin incubation, alpha-BTX was bound to approximately 90% of total available endplate sites. When, without implying localization, the presynaptic axonal membrane was used as a convenient reference structure, the concentration of alpha-BTX relative to this membrane was determined to be 46,000 +/- 27% sites/mum2.
摘要
通过电子显微镜放射自显影术，测定了小鼠胸锁乳突肌中125I-α-银环蛇毒素（α-BTX）结合位点以及乙酰胆碱受体（AChR）的分布和定量。我们发现，神经肌肉接头处受体饱和的一个有效标准是完全消除神经诱发的强直性肌肉收缩，因为当将此标准用于毒素孵育终点时，α-BTX与约90%的总可用终板位点结合。在不考虑定位的情况下，当将突触前轴突膜用作方便的参考结构时，相对于该膜的α-BTX浓度测定为46,000±27%位点/μm²。
相似文献
1
Quantitation of junctional and extrajunctional acetylcholine receptors by electron microscope autoradiography after 125I-alpha-bungarotoxin binding at mouse neuromuscular junctions.在小鼠神经肌肉接头处进行¹²⁵I-α-银环蛇毒素结合后，通过电子显微镜放射自显影法定量测定接头处和接头外乙酰胆碱受体。
J Cell Biol. 1976 Apr;69(1):144-58. doi: 10.1083/jcb.69.1.144.
2
Localization of acetylcholine receptor by 125I-labeled alpha-bungarotoxin binding at mouse motor endplates.通过125I标记的α-银环蛇毒素在小鼠运动终板处结合来定位乙酰胆碱受体。
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1376-8. doi: 10.1073/pnas.71.4.1376.
3
Denervation increases turnover rate of junctional acetylcholine receptors.去神经支配会增加接头处乙酰胆碱受体的周转率。
Proc Natl Acad Sci U S A. 1980 Apr;77(4):2293-7. doi: 10.1073/pnas.77.4.2293.
4
Distribution of acetylcholine receptors at frog neuromuscular junctions with a discussion of some physiological implications.蛙神经肌肉接头处乙酰胆碱受体的分布及其一些生理学意义的探讨。
J Physiol. 1978 Jun;279:197-213. doi: 10.1113/jphysiol.1978.sp012340.
5
Fine structural distribution of acetylcholine receptors at developing mouse neuromuscular junctions.发育中小鼠神经肌肉接头处乙酰胆碱受体的精细结构分布
J Neurosci. 1983 Mar;3(3):644-57. doi: 10.1523/JNEUROSCI.03-03-00644.1983.
6
Cytochemical localization of acetylcholine receptors at the neuromuscular junction by means of horseradish peroxidase-labeled alpha-bungarotoxin.利用辣根过氧化物酶标记的α-银环蛇毒素对神经肌肉接头处乙酰胆碱受体进行细胞化学定位。
Brain Res. 1977 Sep 2;132(3):423-42. doi: 10.1016/0006-8993(77)90192-5.
7
In vivo recovery of muscle contraction after alpha-bungarotoxin binding.α-银环蛇毒素结合后肌肉收缩的体内恢复情况
J Cell Biol. 1975 Jul;66(1):209-13. doi: 10.1083/jcb.66.1.209.
8
Acetylcholine receptor and ion conductance modulator sites at the murine neuromuscular junction: evidence from specific toxin reactions.小鼠神经肌肉接头处的乙酰胆碱受体和离子电导调节位点：来自特定毒素反应的证据。
Proc Natl Acad Sci U S A. 1973 Mar;70(3):949-53. doi: 10.1073/pnas.70.3.949.
9
Loss of alpha-bungarotoxin from junctional and extrajunctional acetylcholine receptors in rat diaphragm muscle in vivo and in organ culture.大鼠膈膜肌体内及器官培养中，接头处和接头外乙酰胆碱受体的α-银环蛇毒素丧失。
J Physiol. 1975 Nov;252(3):771-89. doi: 10.1113/jphysiol.1975.sp011169.
10
Ultrastructural studies on the acetylcholine receptor at motor end plates of normal and pathologic muscles.正常和病理肌肉运动终板处乙酰胆碱受体的超微结构研究。
Ann N Y Acad Sci. 1976;274:85-107. doi: 10.1111/j.1749-6632.1976.tb47678.x.
引用本文的文献
1
Modulation of a rapid neurotransmitter receptor-ion channel by membrane lipids.膜脂对快速神经递质受体-离子通道的调节作用。
Front Cell Dev Biol. 2024 Jan 11;11:1328875. doi: 10.3389/fcell.2023.1328875. eCollection 2023.
2
Fluorescence microscopy imaging of a neurotransmitter receptor and its cell membrane lipid milieu.神经递质受体及其细胞膜脂质环境的荧光显微镜成像。
Front Mol Biosci. 2022 Nov 28;9:1014659. doi: 10.3389/fmolb.2022.1014659. eCollection 2022.
3
Activity-regulated E3 ubiquitin ligase TRIM47 modulates excitatory synapse development.活性调节的E3泛素连接酶TRIM47调节兴奋性突触的发育。
Front Mol Neurosci. 2022 Sep 21;15:943980. doi: 10.3389/fnmol.2022.943980. eCollection 2022.
4
"Calcium bombs" as harbingers of synaptic pathology and their mitigation by magnesium at murine neuromuscular junctions.“钙炸弹”作为突触病理学的预兆及其在小鼠神经肌肉接头处被镁缓解的情况。
Front Mol Neurosci. 2022 Jul 26;15:937974. doi: 10.3389/fnmol.2022.937974. eCollection 2022.
5
Membraneless condensates by Rapsn phase separation as a platform for neuromuscular junction formation.无膜凝聚物通过 Rapsn 相分离作为神经肌肉接头形成的平台。
Neuron. 2021 Jun 16;109(12):1963-1978.e5. doi: 10.1016/j.neuron.2021.04.021. Epub 2021 May 24.
6
Impairment Mechanisms and Intervention Approaches for Aged Human Neuromuscular Junctions.老年人神经肌肉接头的损伤机制与干预方法
Front Mol Neurosci. 2020 Nov 16;13:568426. doi: 10.3389/fnmol.2020.568426. eCollection 2020.
7
HDAC6 regulates microtubule stability and clustering of AChRs at neuromuscular junctions.组蛋白去乙酰化酶 6 调控神经肌肉接头处的微管稳定性和乙酰胆碱受体的聚集。
J Cell Biol. 2020 Aug 3;219(8). doi: 10.1083/jcb.201901099.
8
Site-directed MT1-MMP trafficking and surface insertion regulate AChR clustering and remodeling at developing NMJs.定点定向 MT1-MMP 转运和表面插入调节发育中的 NMJs 处的 AChR 聚集和重塑。
Elife. 2020 Mar 24;9:e54379. doi: 10.7554/eLife.54379.
9
Zebrafish neuromuscular junction: The power of N.斑马鱼神经肌肉接头：神经的力量
Neurosci Lett. 2019 Nov 20;713:134503. doi: 10.1016/j.neulet.2019.134503. Epub 2019 Sep 23.
10
Vezatin is required for the maturation of the neuromuscular synapse.Vezatin 对于神经肌肉突触的成熟是必需的。
Mol Biol Cell. 2019 Sep 15;30(20):2571-2583. doi: 10.1091/mbc.E19-06-0313. Epub 2019 Aug 14.
本文引用的文献
1
Substrate concentration and specificity of choline ester-splitting enzymes.胆碱酯裂解酶的底物浓度和特异性
Arch Biochem. 1949 Aug;23(1):111-26.
2
AUTORADIOGRAPHY WITH THE ELECTRON MICROSCOPE. A PROCEDURE FOR IMPROVING RESOLUTION, SENSITIVITY, AND CONTRAST.电子显微镜放射自显影术。一种提高分辨率、灵敏度和对比度的方法。
J Cell Biol. 1964 Aug;22(2):469-77. doi: 10.1083/jcb.22.2.469.
3
ISOLATION OF NEUROTOXINS FROM THE VENOM OF BUNGARUS MULTICINCTUS AND THEIR MODES OF NEUROMUSCULAR BLOCKING ACTION.从银环蛇毒液中分离神经毒素及其神经肌肉阻断作用方式
Arch Int Pharmacodyn Ther. 1963 Jul 1;144:241-57.
4
The fine structure of the neuromuscular junction of the frog.青蛙神经肌肉接头的精细结构。
J Physiol. 1960 Jan;150(1):134-44. doi: 10.1113/jphysiol.1960.sp006378.
5
The ultrastructure of a reptilian myoneural junction.一种爬行动物肌神经接头的超微结构。
J Biophys Biochem Cytol. 1956 Jul 25;2(4):381-94. doi: 10.1083/jcb.2.4.381.
6
Electron microscopy of the motor end-plate in rat intercostal muscle.大鼠肋间肌运动终板的电子显微镜检查。
Anat Rec. 1955 May;122(1):1-15. doi: 10.1002/ar.1091220102.
7
Localization of the cholinergic receptor protein in Electrophorus electroplax by high resolution autoradiography.通过高分辨率放射自显影术对电鳗电板中胆碱能受体蛋白进行定位。
FEBS Lett. 1972 Sep 1;25(1):127-133. doi: 10.1016/0014-5793(72)80469-1.
8
Electron microscope radioautography as a quantitative tool in enzyme cytochemistry. I. The distribution of acetylcholinesterase at motor end plates of a vertebrate twitch muscle.电子显微镜放射自显影术作为酶细胞化学中的一种定量工具。I. 脊椎动物快肌运动终板处乙酰胆碱酯酶的分布。
J Cell Biol. 1967 Feb;32(2):379-89. doi: 10.1083/jcb.32.2.379.
9
Electron microscope radioautography as a quantitative tool in enzyme cytochemistry. II. The distribution of DFP-reactive sties at motor endplates of a vertebrate twitch muscle.电子显微镜放射自显影术作为酶细胞化学中的一种定量工具。II. 脊椎动物快肌运动终板处二异丙基氟磷酸酯反应位点的分布
J Cell Biol. 1969 Jul;42(1):122-34. doi: 10.1083/jcb.42.1.122.
10
Synthesis, storage and release of [14C]acetylcholine in isolated rat diaphragm muscles.[14C]乙酰胆碱在离体大鼠膈肌中的合成、储存及释放
J Physiol. 1970 Jan;206(1):145-66. doi: 10.1113/jphysiol.1970.sp009003.
Zotero 插件