一种用于测定微粒体苯乙烯单加氧酶和苯乙烯环氧化物水合酶活性的特定气相色谱法。
A specific gas chromatographic method for the determination of microsomal styrene monooxygenase and styrene epoxide hydratase activities.
作者信息
Belvedere G, Pachecka J, Cantoni L, Mussini E, Salmona M
出版信息
J Chromatogr. 1976 Apr 7;118(3):387-93. doi: 10.1016/s0021-9673(00)82176-7.
PMID:1254672
Abstract
A gas chromatographic (GC) method for the determination of the metabolite resulting from the activities of microsomal styrene monooxygenase (epoxide synthetase) and epoxide hydratase using styrene or styrene epoxide as substrates has been developed. The determination of the activities of both enzymes is based on the GC determination of phenylethylene glycol after its esterification with n-butylboronic acid. Kinetic parameters for both enzymes are given.
摘要
已开发出一种气相色谱（GC）方法，用于测定以苯乙烯或苯乙烯环氧化物为底物时，微粒体苯乙烯单加氧酶（环氧化物合成酶）和环氧化物水合酶活性产生的代谢物。两种酶活性的测定基于苯乙二醇与正丁基硼酸酯化后通过气相色谱进行的测定。给出了两种酶的动力学参数。
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