Romano M, Garattini E, Salmona M
Dev Pharmacol Ther. 1985;8(4):232-42. doi: 10.1159/000457042.
The development of cytochrome P-450, cytochrome C reductase, aryl hydrocarbon hydroxylase, styrene monooxygenase, and styrene epoxide hydrolase was studied in fresh rabbit liver nuclear and microsomal preparations. Enzymatic activities were determined in rabbit fetus at 21 and 30 days of gestation and in rabbits at 1, 5, 11, 22, 44, and 60 days of age. In extrauterine life, nuclear cytochrome P-450, aryl hydrocarbon hydroxylase, and styrene epoxide hydrolase were found to develop later than their microsomal counterparts, whereas nuclear cytochrome C reductase developed earlier than its microsomal counterpart. The behavior of styrene monooxygenase was more complex. The only activities detectable before birth were styrene epoxide hydrolase, styrene monooxygenase, and cytochrome C reductase in both nuclei and microsomes. The microsomes-to-nuclei epoxide hydrolase activity ratio was almost ten times the microsomes-to-nuclei styrene monooxygenase ratio, indicating a dissociation in the development of the two enzymes in rabbits. Nuclear metabolic activity was high at all ages, suggesting possible toxicological relevance.
在新鲜兔肝细胞核和微粒体制剂中研究了细胞色素P - 450、细胞色素C还原酶、芳烃羟化酶、苯乙烯单加氧酶和苯乙烯环氧化物水解酶的发育情况。测定了妊娠21天和30天的兔胎儿以及1、5、11、22、44和60日龄兔的酶活性。在出生后的生命中，发现细胞核细胞色素P - 450、芳烃羟化酶和苯乙烯环氧化物水解酶的发育比微粒体中的对应酶晚，而细胞核细胞色素C还原酶的发育比微粒体中的对应酶早。苯乙烯单加氧酶的行为更为复杂。出生前唯一可检测到的活性是细胞核和微粒体中的苯乙烯环氧化物水解酶、苯乙烯单加氧酶和细胞色素C还原酶。微粒体与细胞核的环氧化物水解酶活性比几乎是微粒体与细胞核苯乙烯单加氧酶活性比的十倍，这表明兔体内这两种酶的发育存在解离。在所有年龄段，细胞核代谢活性都很高，这表明可能具有毒理学相关性。
