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一种用于保存混合淋巴细胞反应中白细胞刺激功能的简单冷冻和储存方法。

A simple freezing and storage method to preserve the stimulating function of leukocytes in the mixed leukocyte reaction.

作者信息

Osoba D, Falk J, Dajko M, Richardson J

出版信息

Tissue Antigens. 1975 May;5(4):226-33. doi: 10.1111/j.1399-0039.1975.tb01468.x.

DOI:10.1111/j.1399-0039.1975.tb01468.x
PMID:125468
Abstract

Human peripheral blood leukocytes retain their capacity to function as stimulating cells in the one way MLR after freezing and storage at - 70 degrees C by a simple and inexpensive method. This storage method facilitates the use of a standard stimulating pool of cells from three donors which may provide a quantitative assay for testing the ability of cells to respond to allogeneic cells in culture.

摘要

通过一种简单且经济的方法，人外周血白细胞在冷冻并储存于-70℃后，仍保留其在单向混合淋巴细胞反应中作为刺激细胞的功能。这种储存方法便于使用来自三名供体的标准刺激细胞库，这可能为测试细胞在培养中对异基因细胞的反应能力提供一种定量检测方法。

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A simple freezing and storage method to preserve the stimulating function of leukocytes in the mixed leukocyte reaction.一种用于保存混合淋巴细胞反应中白细胞刺激功能的简单冷冻和储存方法。
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