Caspi R R, Avtalion R R
Dev Comp Immunol. 1984 Summer;8(3):631-7. doi: 10.1016/0145-305x(84)90095-8.
Two-way and one-way mixed leukocyte reaction (MLR) was demonstrated in peripheral blood leukocytes (PBL) of carp. Primary two-way MLR in randomly selected donor pairs were highly variable. Weak primary responses could be strongly augmented by mutual in vivo priming of the reacting donors. One-way MLR was performed using irradiated (16,000 R) allogeneic PBL as stimulators. Reciprocal responses of randomly paired donors were usually unequal, suggesting the usefulness of this method for genetic analysis of MLR-recognized histocompatibility antigens in carp. Kinetics of the primary and secondary two-way MLR were studied, as well as the kinetics of primary one-way MLR against pooled allogenic stimulator cells.
在鲤鱼外周血白细胞（PBL）中证实了双向和单向混合淋巴细胞反应（MLR）。随机选择的供体对之间的原发性双向MLR具有高度变异性。反应性供体的体内相互致敏可强烈增强微弱的原发性反应。单向MLR使用经照射（16,000 R）的同种异体PBL作为刺激物进行。随机配对供体的相互反应通常不相等，这表明该方法可用于鲤鱼中MLR识别的组织相容性抗原的遗传分析。研究了原发性和继发性双向MLR的动力学，以及针对混合同种异体刺激细胞的原发性单向MLR的动力学。
