香豆素对线粒体功能的影响I.氧化磷酸化的解偶联作用

Effect of coumarins on mitochondrial function I. Uncoupling of oxidative phosphorlation.

作者信息

Ramachandra Pai M, Bai N J, Venkitasubramania T A, Murthy V V

出版信息

Toxicology. 1975 Jul;4(3):297-303. doi: 10.1016/0300-483x(75)90052-9.

Abstract

8-Acetyl-5,7-diemthoxy-4-phenylcoumarin at concentrations 0.03 to 0.3 mM uncoupled oxidative phosphorylation, in vitro, in rat liver mitochondria. Preincubation of mitochondria with the compound enhanced this effct. Similar uncoupling was observed with 5,7-dihydroxy-4phenylcoumarin; 7-acetonyloxycoumarin; 6,7-dimethyl-4-phenylcoumarin and 4-phenyldaphentin also. All these compounds stimulated mitochondrial ATPase activity three- to eight-fold. However, coumarin, the paretn substance of all these compounds had no effect on oxidative phosphorylation in mitochondria.

摘要

8-乙酰基-5,7-二甲氧基-4-苯基香豆素在浓度为0.03至0.3 mM时，可在体外使大鼠肝线粒体的氧化磷酸化解偶联。线粒体与该化合物预孵育可增强这种效应。5,7-二羟基-4-苯基香豆素、7-丙酮氧基香豆素、6,7-二甲基-4-苯基香豆素和4-苯基连苯三酚也观察到类似的解偶联现象。所有这些化合物均可使线粒体ATP酶活性提高三至八倍。然而，所有这些化合物的母体物质香豆素对线粒体中的氧化磷酸化没有影响。