Walsh R J, Reinot T, Hayes J M, Kalli K R, Hartmann L C, Small G J
Ames Laboratory--USDOE, Iowa State University, Ames, Iowa 50011, USA.
Biophys J. 2003 Feb;84(2 Pt 1):1299-307. doi: 10.1016/S0006-3495(03)74945-5.
Results are presented of nonphotochemical-hole-burning experiments on the mitochondrial specific dye rhodamine 800 incubated with two human ovarian surface epithelial cell lines: OSE(tsT)-14 normal cells and OV167 carcinoma cells. This dye is selective for the plasma and inner membranes of the mitochondria, as shown by confocal microscopy images. Dispersive hole-growth kinetics of zero-phonon holes are analyzed with theoretical fits, indicating that subcellular structural heterogeneity of the carcinoma cell line is lower relative to the analogous normal cell line. Broadening of holes in the presence of an applied electric field (Stark effect) was used to determine the permanent dipole moment change for the S(0)-->S(1) transition in the two cell lines. For the carcinoma cell line, the permanent dipole moment change value is a factor of 1.5 higher than for the normal cell line. It is speculated that this difference may be related to differences in mitochondrial membrane potentials in the two cell lines.
本文展示了用两种人类卵巢表面上皮细胞系（OSE(tsT)-14正常细胞和OV167癌细胞）培养的线粒体特异性染料罗丹明800的非光化学空穴烧蚀实验结果。共聚焦显微镜图像显示，这种染料对线粒体的质膜和内膜具有选择性。用理论拟合分析了零声子空穴的色散空穴生长动力学，结果表明，癌细胞系的亚细胞结构异质性低于类似的正常细胞系。利用外加电场存在时空穴的展宽（斯塔克效应）来确定两种细胞系中S(0)→S(1)跃迁的永久偶极矩变化。对于癌细胞系，永久偶极矩变化值比正常细胞系高1.5倍。据推测，这种差异可能与两种细胞系中线粒体膜电位的差异有关。
