Geiser Marianne, Schurch Samuel, Gehr Peter
Institute of Anatomy, University of Bern, Bern, Switzerland.
J Appl Physiol (1985). 2003 May;94(5):1793-801. doi: 10.1152/japplphysiol.00514.2002. Epub 2003 Jan 24.
Inhaled and deposited spherical particles, 1-6 micrometer in diameter and of differing surface chemistry and topography, were studied in hamster intrapulmonary conducting airways and alveoli by electron microscopy. Polystyrene and Teflon particles, as well as puffball spores, were found submersed in the aqueous lining layer and adjacent to epithelial cells. The extent of particle immersion promoted by a surfactant film was assessed in a "floating-drop-surface balance" by light microscopy. Teflon and polystyrene spheres were immersed into the subphase by 50-60% at film surface tensions of 25 and 30 mJ/m(2), respectively, and totally submersed at 15 and 25 mJ/m(2), respectively. Puffball spores were immersed by approximately 50% at 22 mJ/m(2) and totally submersed at film surface tensions of </=15 mJ/m(2). These results suggest that the surface tension in the intrapulmonary conducting airways of hamsters may reach </=15 mJ/m(2) and that respirable particles (<10 micrometer in diameter) are wetted and displaced into the surface lining layer, which may facilitate interactions with many lung cells.
通过电子显微镜，对直径为1 - 6微米、具有不同表面化学性质和形貌的吸入并沉积在仓鼠肺内传导气道和肺泡中的球形颗粒进行了研究。发现聚苯乙烯和聚四氟乙烯颗粒以及马勃菌孢子浸没在水衬层中并靠近上皮细胞。通过光学显微镜在“浮滴表面天平”中评估了表面活性剂膜促进颗粒浸没的程度。在膜表面张力分别为25和30 mJ/m²时，聚四氟乙烯和聚苯乙烯球体分别有50 - 60%浸没到亚相，在15和25 mJ/m²时则完全浸没。马勃菌孢子在22 mJ/m²时约有50%浸没，在膜表面张力≤15 mJ/m²时完全浸没。这些结果表明，仓鼠肺内传导气道中的表面张力可能达到≤15 mJ/m²，并且可吸入颗粒（直径<10微米）会被湿润并移入表面衬层，这可能有助于与许多肺细胞相互作用。
