Turley R B, Kloth R H
USDA-ARS, Crop Genetics and Production Research, P. O. Box 345, Stoneville, MS 38776-0345, USA.
J Hered. 2002 Sep-Oct;93(5):359-64. doi: 10.1093/jhered/93.5.359.
Segregating populations were developed to evaluate the inheritance of the fuzzless seed phenotypes in upland cotton (Gossypium hirsutum L.). Accession 143 of the Mississippi Obsolete Variety Collection (MOVC) has a fuzzless seed phenotype. This line carries the n(2) locus which is recessive to the seed fuzz phenotype. Data from the F(2), BC(1)F(1), F(2:3), and BC(1)F(2) populations of DP 5690 x 143 fit a two-loci model for expression of the recessive fuzzless seed phenotype. Fuzzless seeds were obtained in n(2)n(2) plants when a second recessive locus (n(3)) was present. The dominant N(3) allele found in DP 5690 confers the fuzzy seed phenotype in homozygous n(2) plants. Accession 243 of the MOVC carries the N(1) locus, which is dominant to the presence of seed coat fuzz. No variation from expected ratios was observed in the F(2), BC(1)F(1), F(2:3), and BC(1)F(2) populations of the DP 5690 x 243 cross. The N(3) allele had no apparent effect on the expression of the N(1) locus. In a cross between accessions 243 x 143, a few plants were observed which were completely devoid of lint and fuzz fiber (fiberless). A fiberless line was developed from one of these fiberless plants. This line was designated MD 17 fiberless. In a cross between DP 5690 x MD 17 fiberless, we demonstrated that at least three loci were involved in the expression of the fiberless phenotype. The involvement of n(2) and n(3) in the expression of this fiberless phenotype was demonstrated in the F(2) progeny of the cross between 143 x MD 17 fiberless. This is the first demonstration that N(1), n(2), and n(3) interacted to produce fiberless seed.
构建了分离群体以评估陆地棉（Gossypium hirsutum L.）无毛籽表型的遗传情况。密西西比过时品种收集库（MOVC）的143号种质具有无毛籽表型。该品系携带n(2)位点，其对有籽棉表型为隐性。DP 5690×143的F(2)、BC(1)F(1)、F(2:3)和BC(1)F(2)群体的数据符合隐性无毛籽表型表达的双位点模型。当存在第二个隐性位点（n(3)）时，在n(2)n(2)植株中获得了无毛籽。DP 5690中发现的显性N(3)等位基因在纯合n(2)植株中赋予有籽棉表型。MOVC的243号种质携带N(1)位点，其对种皮有棉毛为显性。在DP 5690×243杂交的F(2)、BC(1)F(1)、F(2:3)和BC(1)F(2)群体中未观察到与预期比例的差异。N(3)等位基因对N(1)位点的表达没有明显影响。在243×143种质之间的杂交中，观察到一些完全没有棉绒和棉纤维（无纤维）的植株。从这些无纤维植株之一培育出了一个无纤维品系。该品系被命名为MD 17无纤维。在DP 5690×MD 17无纤维的杂交中我们证明，至少三个位点参与了无纤维表型的表达。在143×MD 17无纤维杂交的F(2)后代中证明了n(2)和n(3)参与了这种无纤维表型的表达。这是首次证明N(1)、n(2)和n(3)相互作用产生无纤维种子。
