Cotton Fiber Bioscience Research Unit, USDA-ARS-SRRC, New Orleans, LA, 70124, USA.
Cotton Chemistry and Utilization Research Unit, USDA-ARS-SRRC, New Orleans, LA, 70124, USA.
Mol Genet Genomics. 2021 Sep;296(5):1041-1049. doi: 10.1007/s00438-021-01802-0. Epub 2021 Jun 10.
Cotton fiber mutants are valuable resources for studying functions of altered genes and their roles in fiber development. The n is a recessive tufted-fuzzless seed mutant created through chemical mutagenesis with ethyl methanesulfonate. Genetic analysis indicated that the tufted-fuzzless phenotype is controlled by a single recessive locus. In this study, we developed an F population of 602 progeny plants and sequenced the genomes of the parents and two DNA bulks from F progenies showing the mutant phenotype. We identified DNA sequence variants between the tufted-fuzzless mutant and wild type by aligning the sequence reads to the reference TM-1 genome and designed subgenome-specific SNP markers. We mapped the n locus on chromosome D04 within a genomic interval of about 411 kb. In this region, seven genes showed significant differential expression between the tufted-fuzzless mutant and wild type. Possible candidate genes are discussed in this study. The utilization of the n mutant along with other fiber mutants will facilitate our understanding of the molecular mechanisms of cotton fiber cell growth and development.
棉花纤维突变体是研究基因功能及其在纤维发育中作用的宝贵资源。n 是一个隐性的丛生无绒毛种子突变体，是通过乙基甲磺酸乙酯化学诱变产生的。遗传分析表明，丛生无绒毛表型由单个隐性基因座控制。在这项研究中，我们开发了一个由 602 株后代组成的 F 群体，并对亲本和两个表现突变表型的 F 后代 DNA 进行了测序。我们通过将序列读取与参考 TM-1 基因组进行比对，鉴定了突变体和野生型之间的 DNA 序列变异，并设计了亚基因组特异性 SNP 标记。我们将 n 基因定位在 D04 染色体上，位于约 411kb 的基因组区间内。在这个区域，有七个基因在丛生无绒毛突变体和野生型之间表现出显著的差异表达。本研究讨论了可能的候选基因。该突变体与其他纤维突变体的利用将有助于我们理解棉花纤维细胞生长和发育的分子机制。
