Chen Z Y, Huang A J, Bao X, Lu C L
Department of Neurobiology, Second Military Medical University, Shanghai 200433.
Shi Yan Sheng Wu Xue Bao. 2000 Sep;33(3):237-44.
The effect of GDNF on long-term cultured spinal cord neurons was studied. GDNF could promote spinal cord neurons survival after 7 d or 14 d culture by MTT assay. The effect of GDNF on growth cones, neuron soma magnitude, neurite length and spines formulation of spinal cord neurons in cell culture was observed by phase microscopy, Nissl stain and NSE immunocytochemistry stain. The results indicated that GDNF had significant trophic effects on long-term cultured spinal cord neurons.
研究了胶质细胞源性神经营养因子（GDNF）对长期培养的脊髓神经元的影响。通过MTT法检测发现，GDNF可促进培养7天或14天后脊髓神经元的存活。采用相差显微镜、尼氏染色和神经元特异性烯醇化酶（NSE）免疫细胞化学染色观察GDNF对细胞培养中脊髓神经元生长锥、神经元胞体大小、神经突长度和棘突形成的影响。结果表明，GDNF对长期培养的脊髓神经元具有显著的营养作用。
